GenePage for the tsaC gene of Escherichia coli K-12

Primary Gene Name: tsaC
EcoGene Accession Number: EG12840
K-12 Gene Accession Number: ECK3269
MG1655 Gene Identifier: b3282
Gene Name Mnemonic: Threonylcarbamoyl-6(Six)-Adenosine
Alternate Gene Symbols: rimN; yrdC
Description: tRNA(NNU) t(6)A37 threonylcarbamoyladenosine modification; threonine-dependent ADP-forming ATPase
  # bp Upstream # bp Downstream
MW: 20767.74 ---------190 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 3430843
Left Intergenic Region

Name: aroE_tsaC

Length: 4 bp gap

Orientation: Codirectional-

Left_end: 3430839

Right_end: 3430842

Centisome: 73.91

Genomic Address
Counterclockwise
Minute or Centisome (%) = 73.91
Right End: 3431415
Right Intergenic Region

Name: tsaC_yrdD

Length: 4 bp gap

Orientation: Codirectional-

Left_end: 3431416

Right_end: 3431419

Centisome: 73.93

The modified tRNA nucleotide t(6)A37 is derived from the labile cyclized ct(6)A37 during extraction; the tRNA threonylcarbamoyladenosine dehydratase TcdA catalyzes the cyclization reaction (Miyauchi, 2013). TsaBCDE are necessary and sufficient for tRNA(NNU) t(6)A37 threonylcarbamoyladenosine modification in vitro (Deutsch, 2012). TsaC(YrdC) has in vitro threonine-dependent ATPase activity generating AMP (Deutsch, 2012). TsaD(YgjD) was proposed to be a threonine-bicarbonate ligase producing N-carbamoylthreonine which was proposed to then be transferred to the N(6) group of tRNA(NNU) A37 by TsaC(YrdC) (El Yacoubi, 2011). TsaC(YrdC) but not paralog YciO is required for synthesis of t(6)A37 threonylcarbamoyladenosine of ANN decoding tRNA(NNU)s (El Yacoubi, 2009). TsaA paralog YciO does not complement a tsaC(yrdC) mutational defect in t(6)A37 synthesis (Gerdes, 2011). TsaC(YrdC) efficiently binds to unmodified but not modified uncharged tRNA (El Yacoubi, 2009; Harris, 2011). TsaC(YrdC) was proposed to be a threonylcarbamoyl transferase subunit and part of a heteromultimeric complex as it binds unmodified tRNA, threonine and ATP (Harris, 2011). A tsaC(yrdC) deletion suppresses a prfA mutation and can only survive in a prfA mutant. tsaC(yrdC) mutants were observed to be defective in ribosome maturation and was named rimN, but this could be a secondary effect due to its essential role in tRNA(NNU) t(6)A37 modification (Kaczanowska, 2005).

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BamHI EcoRI HindIII