GenePage for the pqiB gene of Escherichia coli K-12

Primary Gene Name: pqiB
EcoGene Accession Number: EG12966
K-12 Gene Accession Number: ECK0942
MG1655 Gene Identifier: b0951
Gene Name Mnemonic: Paraquat-inducible
Alternate Gene Symbols: pqi5
Description: Periplasmic MCE subunit of the putative PqiABC transporter; IM-anchored; paraquat-inducible, SoxRS-regulated; RpoS regulon
  # bp Upstream # bp Downstream
MW: 60520.47 ---------546 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 1013259
Left Intergenic Region

Name: pqiA_pqiB

Length: 4 bp gap

Orientation: Codirectional+

Left_end: 1013255

Right_end: 1013258

Centisome: 21.83

Genomic Address
Clockwise
Minute or Centisome (%) = 21.83
Right End: 1014899
Right Intergenic Region

Name: pqiB_pqiC

Length: 4 bp overlap

Orientation: Codirectional+

Left_end: 1014896

Right_end: 1014899

Centisome: 21.86

Overexpression of plasmid-encoded pqiABC suppresses the SDS(0.1%)/EDTA(0.95 mM) sensitivity of an mlaD deletion strain, inferring a role for PqiABC in maintaining outer membrane (OM) integrity, probably by being a transport pathway functionally similar to the Mla transport pathway (which maintains OM asymmetry by removing phospholipids from the OM outer leaflet); simultaneous deletion of pqiB and yebT in a mlaD mutant background increases sensitivity to SDS/EDTA, a phenotype complemented weakly by a pqiBC plasmid and more fully by a pqiABC plasmid; PqiB was localized to the periplasm by a protease accessibility assay; yeast two-hybrid assays were used to demonstrate PqiB homo-oligimerization and PqiB-PqiC protein-protein interaction (Nakayama, 2016). The pqiABC operon P1 promoter is paraquat-inducible (superoxide generator-inducible) and SoxRS-regulated; P2 is RpoS-regulated (Koh, 1995; Kim, 2005; Ito, 2008). PqiB has three tandem MCE/MlaD (mammalian cell entry, Pfam:PF02470, InterPro:IPR003399) domains; PqiB has a possible (E=3e-03) C-terminal DASH complex subunit Duo1 PF08651 domain followed by a possible coil-coil or disordered region that may be involved in homodimerization and/or a periplasmic interaction with PqiC. TMHMM strongly predicts that residues 16-38 of PqiB comprise a single transmembrane region oriented such that the bulk of the protein is in the periplasm. The pqiAB and yebST genes are paralogous.

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BamHI EcoRI HindIII