GenePage for the pbl' pseudogene of Escherichia coli K-12

Primary Gene Name: pbl'
EcoGene Accession Number: EG13040
K-12 Gene Accession Number: ECK2852
MG1655 Gene Identifier: b2854
Gene Name Mnemonic: Peptidoglycan-binding like
Alternate Gene Symbols: ygeJ
Description: Pseudogene reconstruction, pilT homolog; part of T3SS PAI ETT2 remnant; putative murein hydrolase; putative periplasmic protein
  # bp Upstream # bp Downstream
MW: 19348.62 ---------167 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 2993939
Left Intergenic Region

Name: ygeI_pbl

Length: 82 bp gap

Orientation: Codirectional+

Left_end: 2993857

Right_end: 2993938

Centisome: 64.50

Genomic Address join

The following join statement uses GenBank syntax to describe how the reconstructed frameshifted pbl pseudogene protein sequence was translated from the DNA:

join(2993939..2994382,2994382..2994441)


Clockwise
Minute or Centisome (%) = 64.50
Right End: 2994441
Right Intergenic Region

Name: pbl_ygeK

Length: 18 bp gap

Orientation: Convergent

Left_end: 2994442

Right_end: 2994459

Centisome: 64.51

This pseudogene is a remnant of the type three secretion system (T3SS) pathogenicity island ETT2. 18 kb of ETT2 has been deleted from K-12, leaving 10 kb of the presumably non-functional T3SS ETT2 remaining. Pbl is homologous to virulence-related export proteins, e.g. IagB (S.t.), IpgF (S. flexneri), PilT (plasmid R64). This family may be involved in making holes in the peptidoglycan layer for macromolecular transport purposes. The first 24 aa of Pbl are predicted to be a type I signal peptide. Codon usage indicates pbl is an alien (horizontal) gene. Hypothetical reconstruction of an apparent frameshift mutation after codon 148 extends Pbl to 167 aa. An intact version (Z4175) is present in strain 042 and and in O157:H7 EDL933 (SP Q8X6H3).

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BamHI EcoRI HindIII