GenePage for the ygfK gene of Escherichia coli K-12

Primary Gene Name: ygfK
EcoGene Accession Number: EG13061
K-12 Gene Accession Number: ECK2874
MG1655 Gene Identifier: b2878
Gene Name Mnemonic: Systematic nomenclature
Alternate Gene Symbols: None
Description: Putative selenate reductase FeS subunit; mutants impaired in selenium reduction; NADPH:O2 oxidoreductase activity
  # bp Upstream # bp Downstream
MW: 115581.58 ---------1032 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 3016060
Left Intergenic Region

Name: mocA_ygfK

Length: 321 bp gap

Orientation: Codirectional+

Left_end: 3015739

Right_end: 3016059

Centisome: 64.97

Genomic Address
Clockwise
Minute or Centisome (%) = 64.98
Right End: 3019158
Right Intergenic Region

Name: ygfK_ssnA

Length: 2 bp gap

Orientation: Codirectional+

Left_end: 3019159

Right_end: 3019160

Centisome: 65.04

YgfK contains iron and is an iron–sulfur flavoprotein with in vitro NADPH:O2 oxidoreductase activity (Sevcenco, 2011). A Tn insertion in ygfK is unable to reduce selenate to selenite; YgfKM-XdhD(YgfN) was proposed to be a selenate reductase structural complex but no activity could be detected (Bébien, 2002).

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BamHI EcoRI HindIII