GenePage for the tcdA gene of Escherichia coli K-12

Primary Gene Name: tcdA
EcoGene Accession Number: EG13084
K-12 Gene Accession Number: ECK2808
MG1655 Gene Identifier: b2812
Gene Name Mnemonic: Threonylcarbamoyladenosine dehydratase
Alternate Gene Symbols: csdL; ygdL
Description: tRNA threonylcarbamoyladenosine dehydratase; sulfur acceptor for CsdA, binds CsdE
  # bp Upstream # bp Downstream
MW: 28561.99 ---------268 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 2945036
Left Intergenic Region

Name: csdE_tcdA

Length: 50 bp gap

Orientation: Convergent

Left_end: 2944986

Right_end: 2945035

Centisome: 63.45

Genomic Address
Counterclockwise
Minute or Centisome (%) = 63.45
Right End: 2945842
Right Intergenic Region

Name: tcdA_mltA

Length: 238 bp gap

Orientation: Codirectional-

Left_end: 2945843

Right_end: 2946080

Centisome: 63.47

TcdA(CsdL) is an ATP-dependent dehydratase required to convert t(6)A37 to cyclic t(6)A37 in several tRNA species; the cyclized form of the modified base ct(6)A37 is labile during extraction, explaining why it was descibed as t(6)A37 in earlier studies; ct(6)A37 was determined to contain a cyclized active ester with an oxazolone ring (Miyauchi, 2013). TcdA(CsdL) binds to CsdE, linking TcdA(CsdL) to CsdA; TcdA(CsdL) accepts sulfur from the CsdA donor directly in vitro, but CsdE might act as a sulfur relay protein in vivo (Trotter, 2009). TcdA(CsdL) is similar to the N-terminal domains of MoeB/ThiS protein adenylation/thiocarboxylation enzymes which are E1-like, ubiquitin-activating-like proteins, but TcdA(CsdL) lacks the probable zinc binding site found in MoeB/ThiS family.

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BamHI EcoRI HindIII