GenePage for the ybaZ gene of Escherichia coli K-12

Primary Gene Name: ybaZ
EcoGene Accession Number: EG13254
K-12 Gene Accession Number: ECK0448
MG1655 Gene Identifier: b0454
Gene Name Mnemonic: Systematic nomenclature
Alternate Gene Symbols: None
Description: Excision repair protein; alkyltransferase-like protein ATL; binds and enhances nucleotide excision repair of O(6)-alkylguanine; DNA-binding protein; degenerate Ada/Ogt cysteine methyltransferase paralog
  # bp Upstream # bp Downstream
MW: 14449.54 ---------129 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 475982
Left Intergenic Region

Name: ybaY_ybaZ

Length: 30 bp gap

Orientation: Convergent

Left_end: 475952

Right_end: 475981

Centisome: 10.25

Genomic Address
Counterclockwise
Minute or Centisome (%) = 10.25
Right End: 476371
Right Intergenic Region

Name: ybaZ_ffs

Length: 76 bp gap

Orientation: Divergent

Left_end: 476372

Right_end: 476447

Centisome: 10.26

YbaZ is homologous to methylated-DNA--protein-cysteine methyltransferases Ada and Ogt, but lacks the active site cysteine. YbaZ binds O(6)-alkylguanine residues in DNA and blocks alkyltransferase action; YbaZ does not remove methyl groups from O(6)-methylguanine-containing DNA nor does it have glycosylase or endonuclease activity (Margison, 2007). YbaZ preferentially binds DNA with an abasic site, has base-flipping activity, and binds HelD (Chen, 2008). YbaZ strongly enhances nucleotide excision repair of the large O(6)-alkylguanine adducts that are not good substrates for paralogs Ogt and Ada to remove (Mazon, 2009).

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BamHI EcoRI HindIII