GenePage for the ybbN gene of Escherichia coli K-12

Primary Gene Name: ybbN
EcoGene Accession Number: EG13261
K-12 Gene Accession Number: ECK0486
MG1655 Gene Identifier: b0492
Gene Name Mnemonic: Systematic nomenclature
Alternate Gene Symbols: None
Description: DnaK co-chaperone, thioredoxin-like protein; probable DNA replicase folding chaperone; has SXXC not CXXC motif
  # bp Upstream # bp Downstream
MW: 31791.06 ---------284 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 517425
Left Intergenic Region

Name: fetB_ybbN

Length: 62 bp gap

Orientation: Convergent

Left_end: 517363

Right_end: 517424

Centisome: 11.15

Genomic Address
Minute or Centisome (%) = 11.15
Right End: 518279
Right Intergenic Region

Name: ybbN_ybbO

Length: 60 bp gap

Orientation: Codirectional-

Left_end: 518280

Right_end: 518339

Centisome: 11.17

YbbN is a co-chaperone for both DnaJKGrpE and GroESL(Lin, 2011). YbbN assists in refolding of DnaN, and ybbN mutants produce anucleated cells and have DNA replication defects (Le, 2011). YbbN has two tandem C-terminal TPR-like repeat domains. The function of YbbN as both an thiol-disulfide oxidoreductase and as a chaperone is controversial (Vlamis-Gardikas, 2008): YbbN has been reported to oxidize cysteines of RNase A and to have chaperone activity (Caldas, 2006), but this is partially contradicted by a report that YbbN cannot reduce oxidized insulin and has no chaperone activity on citrate synthase (Pan, 2006). This contradiction is substantially resolved by a followup to the Caldas (2006) study which found no evidence for YbbN oxidoreductase activity in vivo and and agree with Pan (2006) that there is no significant in vitro chaperone activity of YbbN alone on citrate syntase, but there is a strong stimulation of DnaK chaperone activity on citrate syntase, thus YbbN is a DnaK co-chaperone (Kthiri, 2008). YbbN binds DnaK and GroEL; ybbN mutants are sensitive to heat stress but not oxidative stress (Kthiri, 2008). HT_Cmplx38_Cyt: Usg+YbbN. otherPDB (S.typhimurium): 3QDN.

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