GenePage for the frmB gene of Escherichia coli K-12

Primary Gene Name: frmB
EcoGene Accession Number: EG13295
K-12 Gene Accession Number: ECK0352
MG1655 Gene Identifier: b0355
Gene Name Mnemonic: Formaldehyde
Alternate Gene Symbols: yaiM
Description: S-formylglutathione hydrolase, formaldehyde degradation
  # bp Upstream # bp Downstream
MW: 31424.48 ---------277 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 377535
Left Intergenic Region

Name: yaiL_frmB

Length: 223 bp gap

Orientation: Convergent

Left_end: 377312

Right_end: 377534

Centisome: 8.13

Genomic Address
Counterclockwise
Minute or Centisome (%) = 8.13
Right End: 378368
Right Intergenic Region

Name: frmB_frmA

Length: 93 bp gap

Orientation: Codirectional-

Left_end: 378369

Right_end: 378461

Centisome: 8.15

FrmB also has p-nitrophenyl C2-C6 fatty acid ester and a-naphthyl acetate carboxylesterase activities in vitro. The frmRAB operon is negatively regulated by frmR, is induced by formaldehyde, and probably degrades formaldehyde. FrmB is 50% identical to P. denitrificans S-formylglutathione hydrolase. FrmB and YeiG are 50% identical paralogs; double mutant is formaldehyde sensitive; this may explain why an frmB single mutant did not have a formadehyde phenotype when tested on Biolog plates (Herring, 2004). The amino terminal half of FrmB is homologus to the C-terminal half of YieL. S9 peptidase family; YeiG or FrmB are not in MEROPS yet, but it has homologs.

Go to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePage

BamHI EcoRI HindIII