GenePage for the iscU gene of Escherichia coli K-12

Primary Gene Name: iscU
EcoGene Accession Number: EG13395
K-12 Gene Accession Number: ECK2526
MG1655 Gene Identifier: b2529
Gene Name Mnemonic: Iron-sulfur cluster
Alternate Gene Symbols: hsm-5; miaC(S.t.); nifU; yfhN
Description: Iron-sulfur cluster assembly scaffold protein
  # bp Upstream # bp Downstream
MW: 13848.59 ---------128 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 2659903
Left Intergenic Region

Name: iscA_iscU

Length: 16 bp gap

Orientation: Codirectional-

Left_end: 2659887

Right_end: 2659902

Centisome: 57.30

Genomic Address
Counterclockwise
Minute or Centisome (%) = 57.31
Right End: 2660289
Right Intergenic Region

Name: iscU_iscS

Length: 27 bp gap

Orientation: Codirectional-

Left_end: 2660290

Right_end: 2660316

Centisome: 57.31

IscU fails to assemble Fe-S clusters in the presence of sodium citrate due to restricted free iron availability, however Fe-S cluster formation is restored, with sodium citrate present, in the presence of IscA. IscU and IscS form a complex (Agar, 2000; Tokumoto, 2002; Kato, 2002; Prischi, 2010). The IscU-IscS complex is stabilized by a covalent disifide bond between Cys328 of IscS and Cys63 of IscU (Kato, 2002). IscU has been classified as a "complex-orphan" protein that is largely unstructured unless bound to a partner, i.e. IscS (Prischi, 2010).

Go to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePage

BamHI EcoRI HindIII