GenePage for the paoA gene of Escherichia coli K-12

Primary Gene Name: paoA
EcoGene Accession Number: EG13559
K-12 Gene Accession Number: ECK0285
MG1655 Gene Identifier: b0286
Gene Name Mnemonic: Periplasmic aldehyde oxidoreductase
Alternate Gene Symbols: yagT
Description: PaoABC aldehyde oxidoreductase, 2Fe-2S subunit; periplasmic
  # bp Upstream # bp Downstream
MW: 24342.65 ---------229 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 301884
Left Intergenic Region

Name: paoB_paoA

Length: 4 bp overlap

Orientation: Codirectional-

Left_end: 301884

Right_end: 301887

Centisome: 6.50

Genomic Address
Minute or Centisome (%) = 6.50
Right End: 302573
Right Intergenic Region

Name: paoA_yagU

Length: 417 bp gap

Orientation: Divergent

Left_end: 302574

Right_end: 302990

Centisome: 6.52

yagTSRQ have been renamed as paoABCD (S. Leimkuhler, personal communication, 2010). Mutant cells lacking PaoABC are growth-inhibited in the presence of the the aromatic aldehydes cinnamaldehyde, vanillin, or benzaldehyde at pH 4.0, indicating that PaoABC detoxifies aromatic aldehydes (Neumann, 2009). PaoABC has a broad in vitro specificity for aldehyde substrates, but has a preferences for aromatic aldehydes (Neumann, 2009). The active PaoABC heterotrimer, with all cofactors attached, is transported to the periplasm via the Tat translocase, mediated by a single Tat signal peptide on the PaoC subunit; periplasmic proteins PaoB and PaoA do not have their own signal peptides (Neumann, 2009). MocA is the CTP:molybdopterin cytidylyltransferase responsible for the conversion of the Mo-molybdopterin (Mo-MPT) cofactor to the molybdopterin cytosine dinucleotide (MCD) cofactor (Neumann, 2009). Verified Tat substrate: PaoA(YagT) has a predicted Tat-only (Class I) 49 aa signal peptide (Tullman-Ercek, 2007). The internal TTG at codon 23 could alternatively be utilized to make a protein with a predicted 26 aa signal peptide.

Go to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePage