GenePage for the clsB gene of Escherichia coli K-12

Primary Gene Name: clsB
EcoGene Accession Number: EG13671
K-12 Gene Accession Number: ECK0778
MG1655 Gene Identifier: b0789
Gene Name Mnemonic: Systematic nomenclature
Alternate Gene Symbols: ybhO
Description: Cardiolipin synthase 2
  # bp Upstream # bp Downstream
MW: 47633.51 ---------413 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 822498
Left Intergenic Region

Name: ybhN_clsB

Length: 1 bp overlap

Orientation: Codirectional-

Left_end: 822498

Right_end: 822498

Centisome: 17.72

Genomic Address
Counterclockwise
Minute or Centisome (%) = 17.72
Right End: 823739
Right Intergenic Region

Name: clsB_ybhP

Length: 4 bp overlap

Orientation: Codirectional-

Left_end: 823736

Right_end: 823739

Centisome: 17.75

Three paralogous cls genes encoding cardiolipin synthases, clsA(cls), clsB(ybhO) and clsC(ymdC), need to be deleted in order to prevent in vivo cardiolipin synthesis; most studies on the effects of cardiolipin depletion only used clsA(cls) mutant strains that still sythesize cardiolipin (Tan, 2012). ClsB(YbhO) cardiolipin synthase activity was detected in vitro but not in vivo; the physiological significance of in vitro activity was unclear at the time (Guo, 2000). Previous attempts to express ClsB activity in vivo used a low salt medium which inhibits ClsB actvity; cloned gene expression of ClsB in high salt medium resulted in cardiolipin synthesis (Tan, 2012).

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BamHI EcoRI HindIII