GenePage for the pqiC gene of Escherichia coli K-12

Primary Gene Name: pqiC
EcoGene Accession Number: EG13719
K-12 Gene Accession Number: ECK0943
MG1655 Gene Identifier: b0952
Gene Name Mnemonic: Paraquat-inducible
Alternate Gene Symbols: ymbA
Description: Periplasmic lipoprotein of the putative PqiABC transporter; OM-anchored; PqiB likely forms a periplasmic IM-OM bridge with PqiB; paraquat-inducible; SoxRS-regulated; RpoS regulon
  # bp Upstream # bp Downstream
MW: 20634.41 ---------187 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 1014896
Left Intergenic Region

Name: pqiB_pqiC

Length: 4 bp overlap

Orientation: Codirectional+

Left_end: 1014896

Right_end: 1014899

Centisome: 21.86

Genomic Address
Clockwise
Minute or Centisome (%) = 21.86
Right End: 1015459
Right Intergenic Region

Name: pqiC_rmf

Length: 255 bp gap

Orientation: Codirectional+

Left_end: 1015460

Right_end: 1015714

Centisome: 21.88

Overexpression of plasmid-encoded pqiABC suppresses the SDS(0.1%)/EDTA(0.95 mM) sensitivity of an mlaD deletion strain, inferring a role for PqiABC in maintaining outer membrane (OM) integrity, probably by being a transport pathway functionally similar to the Mla transport pathway (which maintains OM asymmetry by removing phospholipids from the OM outer leaflet); simultaneous deletion of pqiB and yebT in a mlaD mutant background rendered cells more sensitive to SDS/EDTA, a phenotype complemented weakly by a pqiBC plasmid and more fully by a pqiABC plasmid; PqiC was localized to the periplasm using PhoA fusions; yeast two-hybrid assays were used to demonstrate PqiC homo-oligimerization and PqiB-PqiC protein-protein interaction (Nakayama, 2016). The pqiABC operon P1 promoter is paraquat-inducible (superoxide generator-inducible) and SoxRS-regulated; P2 is RpoS-regulated (Koh, 1995; Kim, 2005; Ito, 2008). The first 15 aa of PqiC(YmbA) are predicted to be a type II signal peptide. The PFAM DUF330 family has been renamed as the ABC_trans_aux family (PF03886).

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BamHI EcoRI HindIII