GenePage for the gfcD gene of Escherichia coli K-12

Primary Gene Name: gfcD
EcoGene Accession Number: EG13729
K-12 Gene Accession Number: ECK0975
MG1655 Gene Identifier: b0984
Gene Name Mnemonic: Group 4 Capsule
Alternate Gene Symbols: ymcA
Description: Predicted periplasmic protein; required to make O-antigen capsule
  # bp Upstream # bp Downstream
MW: 78687.16 ---------698 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 1045849
Left Intergenic Region

Name: gfcE_gfcD

Length: 45 bp gap

Orientation: Codirectional-

Left_end: 1045804

Right_end: 1045848

Centisome: 22.53

Genomic Address
Counterclockwise
Minute or Centisome (%) = 22.53
Right End: 1047945
Right Intergenic Region

Name: gfcD_gfcC

Length: 1 bp overlap

Orientation: Codirectional-

Left_end: 1047945

Right_end: 1047945

Centisome: 22.58

E. coli K-12 does not produce O-antigen capsule polysaccharide because the cryptic gfcABCDE-etp-etk operon is silenced by IS1D in the promoter region (Peleg, 2005). GfcD is required for O-antigen capsule formation in enteropathogenic E. coli (EPEC) (Peleg, 2005). gfcD(ymcA) and yjbH are paralogs. GfcD has both type I (21 aa) and (slightly better) type II (18 aa) signal peptide predictions by SignalP/LipoP. GfcD is not likely to be a lipoprotein because the lipobox Cys is poorly conserved; many GfcD homologs that lack the essential lipidated Cys are nonetheless annotated as putative lipoproteins.

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BamHI EcoRI HindIII