GenePage for the clsC gene of Escherichia coli K-12

Primary Gene Name: clsC
EcoGene Accession Number: EG13875
K-12 Gene Accession Number: ECK1032
MG1655 Gene Identifier: b1046
Gene Name Mnemonic: Systematic nomenclature
Alternate Gene Symbols: ymdC
Description: Cardiolipin synthase 3, active in stationary phase
  # bp Upstream # bp Downstream
MW: 53665.93 ---------473 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 1106355
Left Intergenic Region

Name: ymdB_clsC

Length: 1 bp gap

Orientation: Codirectional+

Left_end: 1106354

Right_end: 1106354

Centisome: 23.84

Genomic Address
Clockwise
Minute or Centisome (%) = 23.84
Right End: 1107776
Right Intergenic Region

Name: clsC_opgC

Length: 7 bp gap

Orientation: Convergent

Left_end: 1107777

Right_end: 1107783

Centisome: 23.87

ClsC uses PE, not PG, as the phosphatidyl donor to PG to form cardiolipin (Tan, 2012). Three paralogous cls genes encoding cardiolipin synthases, clsA(cls), clsB(ybhO) and clsC(ymdC), need to be deleted in order to prevent in vivo cardiolipin synthesis; most studies on the effects of cardiolipin depletion only used clsA(cls) mutant strains that still sythesize cardiolipin (Tan, 2012). Full activity of ClsC requires co-expression in the same clone with the upstream gene ymdB; ClsC expression alone or clsC and ymdB expresssion from separate plasmids produces only small amounts of cardiolipin; YmdB is suggested to be a stimulatory subunit of a two-component cardiolipin synthase (Tan, 2012). An alternative explanation for the need to co-express ymdB with clsC to get full ClsC activity may be an obligatory translational coupling of ymdB and clsC needed to get efficient translation using the ClsC TTG start codon (K.E.R.) ClsC utilizes a novel mechanism for cardiolipin synthesis in that phosphatidylethanolamine is used as the phosphatidyl donor to phosphatidylglycerol to form cardiolipin; mutating the two conserved phospholipase D superfamily motifs oblated ClsC activity (Tan, 2012). RpoS regulon.

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BamHI EcoRI HindIII