GenePage for the kdgR gene of Escherichia coli K-12

Primary Gene Name: kdgR
EcoGene Accession Number: EG14018
K-12 Gene Accession Number: ECK1826
MG1655 Gene Identifier: b1827
Gene Name Mnemonic: Ketodeoxygluconate
Alternate Gene Symbols: yebP
Description: Transcriptional repressor for the KDG regulon
  # bp Upstream # bp Downstream
MW: 30029.35 ---------263 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 1909308
Left Intergenic Region

Name: yobH_kdgR

Length: 143 bp gap

Orientation: Convergent

Left_end: 1909165

Right_end: 1909307

Centisome: 41.13

Genomic Address
Counterclockwise
Minute or Centisome (%) = 41.13
Right End: 1910099
Right Intergenic Region

Name: kdgR_yebQ

Length: 176 bp gap

Orientation: Divergent

Left_end: 1910100

Right_end: 1910275

Centisome: 41.15

KdgR regulates 2-keto-3-deoxygluconate (KDG) transport and catabolism (Pouyssegur, 1974). KDG is derived from pectin, a common plant heteropolysaccharide, although E. coli K-12 does not secrete pectinases or uptake pectin oligosaccharides and has only the core KDG regulon as compared to plant pathogens like Erwinia chrysanthemi, whose extracellular pectate lyases and intacellular oligogalacturonate lyase breaks pectin down mainly into 5-keto-4-deoxyuronate (DKI) monomers and to a lesser extent polygalacturonase-generated galacturonate; in E. coli K-12 galacturonate uptake and degradation is mediated by the ExuT permease and the UxaABC catabolic enzymes producing KDG, respectively, and DKI is imported by the KdgR-regulated KDG importer KdgT and catabolized to KDG by the KdgR-regulated KduID enyzymes, also producing KDG; KDG is converted to pyruvate and glyceraldehyde-3-phosphate by KdsK and Eda(KdsA) (Rodionov, 2004; Hugouvieux-Cotte-Pattat, 1996). A kdgR-yebQ clone conferred hypersensitivity to trimethoprim, but a yebQ-alone expression clone did not, leading the authors to conclude that overexpression of the transcriptional regulator KdgR was responsible for the Acr-S phenoptype. 2/360 KdgR peptide masses from MS analysis confirm this start codon and indicate that the initial Met is removed (Maillet, 2007).

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BamHI EcoRI HindIII