GenePage for the epmC gene of Escherichia coli K-12

Primary Gene Name: epmC
EcoGene Accession Number: EG14116
K-12 Gene Accession Number: ECK2320
MG1655 Gene Identifier: b2326
Gene Name Mnemonic: EF-P Modification
Alternate Gene Symbols: yfcM
Description: Elongation Factor P Lys34 hydroxylase
  # bp Upstream # bp Downstream
MW: 21145.59 ---------182 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 2444203
Left Intergenic Region

Name: yfcL_epmC

Length: 33 bp gap

Orientation: Codirectional-

Left_end: 2444170

Right_end: 2444202

Centisome: 52.66

Genomic Address
Counterclockwise
Minute or Centisome (%) = 52.66
Right End: 2444751
Right Intergenic Region

Name: epmC_yfcA

Length: 1 bp overlap

Orientation: Codirectional-

Left_end: 2444751

Right_end: 2444751

Centisome: 52.67

epmC(yfcM) mutants do not display the pleiotropic and slow-growth phenotypes observed for epmAB or efp mutants in either E. coli or Salmonella, indicating that the EpmC-mediated modification is is not required for the translation of polyproline-containing proteins, the function of EF-P requiring the EpmAB-mediated lysine34 lysylation (Doerfel, 2012; Bullwinkle, 2012). EpmA(YjeA) and EpmB(YjeK) are required to modify EF-P Lys34 by lysylation of the epsilon amino group; failure to modify EF-P with lysine results in a slow growth defect; EpmB(YjeK) may convert alpha-lysyl-EF-P to beta-lysyl-EF-P (Yanagisawa, 2010). A third and final EF-P post-translation modification enzyme EpmC(YfcM) is identified as EF-P-Lys34 hydroxylase; EpmC(YfcM) acts after beta-lysinylation of Lys34 by EpmA(YjeA) and EpmB(YjeK); EpmC(YfcM) adds an oxygen atom to the C5 position of Lys34 and does not modify the added beta-lysine; YjeA and YjeK were renamed as EpmA and EpmB, respectively (Peil, 2012).

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BamHI EcoRI HindIII