GenePage for the yfcU' pseudogene of Escherichia coli K-12

Primary Gene Name: yfcU'
EcoGene Accession Number: EG14124
K-12 Gene Accession Number: ECK2332
MG1655 Gene Identifier: b4661, b2338, b2337
Gene Name Mnemonic: Systematic nomenclature
Alternate Gene Symbols: yfcT
Description: Pseudogene reconstruction, FimD fimbrial export usher family
  # bp Upstream # bp Downstream
MW: 97327.22 ---------881 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 2452356
Left Intergenic Region

Name: yfcS_yfcU

Length: 19 bp gap

Orientation: Codirectional-

Left_end: 2452337

Right_end: 2452355

Centisome: 52.83

Genomic Address
Counterclockwise
Minute or Centisome (%) = 52.83
Right End: 2455001
Right Intergenic Region

Name: yfcU_yfcV

Length: 81 bp gap

Orientation: Codirectional-

Left_end: 2455002

Right_end: 2455082

Centisome: 52.89

yfcU' is a reconstructed pseudogene in the yfcOPQRU'V cryptic chaperone–usher fimbrial operon; constitutive expression of yfcOPQRU'V in a delta fim background increases adherence to T24 human bladder carcinoma cells tenfold (Korea, 2010). YfcU' is a member of the FimD outer membrane fimbrial subunit export usher family (Pfam PF00577) with N-terminal (Pfam PF13954) and C-terminal (Pfam PF13953) PapC-like chaperone-pilin complex-binding and pilin assembly domains, respectively. yfcU insertions in E. coli B improve fitness in glucose minimal medium, possibly explaining why yfcU' is mutated in K-12 strains (Schneider, 2000). The first 29 aa of YfcU' are predicted to be a cleavable type I signal peptide. The yfcU' stop codon 579 is translated as X in the reconstruction.

Go to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePage

BamHI EcoRI HindIII