GenePage for the yfeW gene of Escherichia coli K-12

Primary Gene Name: yfeW
EcoGene Accession Number: EG14164
K-12 Gene Accession Number: ECK2425
MG1655 Gene Identifier: b2430
Gene Name Mnemonic: Systematic nomenclature
Alternate Gene Symbols: pbp4B
Description: Penicillin binding protein PBP4B; weak DD-carboxypeptidase activity
  # bp Upstream # bp Downstream
MW: 47752.38 ---------434 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 2548102
Left Intergenic Region

Name: murP_yfeW

Length: 4 bp gap

Orientation: Codirectional+

Left_end: 2548098

Right_end: 2548101

Centisome: 54.90

Genomic Address
Clockwise
Minute or Centisome (%) = 54.90
Right End: 2549406
Right Intergenic Region

Name: yfeW_yfeX

Length: 239 bp gap

Orientation: Convergent

Left_end: 2549407

Right_end: 2549645

Centisome: 54.92

A mutation in yfeW(pbp4B) does not affect growth on MurNAc (Jaeger, 2008). yfeW(pbp4B) is probably transcribed as part of the murQP-yfeW operon and from a promoter within murP (Jaeger, 2008). YfeW(Pbp4B) was originally annotated as a 463 aa protein but has been corrected to use a more likely and well-conserved start codon and YfeW is currently annotated as a 434 aa protein; the first 19 aa of the revised protein sequence are predicted to be a signal peptide. YfeW(Pbp4B) was overexpressed and assayed using the 463 aa version with the unlikely 29 aa N-terminal extension; this is why the authors did not detect the predicted signal peptide and possibly why the recombinant Pbp4B was isolated in membrane fractions when it is likely to be a periplasmic protein (Vega, 2006). UPF0214 family, related to ampC. Probable false positive lipoprotein prediction, Cys not conserved. Non-essential.

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BamHI EcoRI HindIII