GenePage for the flgJ gene of Escherichia coli K-12

Primary Gene Name: flgJ
EcoGene Accession Number: EG14272
K-12 Gene Accession Number: ECK1066
MG1655 Gene Identifier: b1081
Gene Name Mnemonic: Flagella
Alternate Gene Symbols: flaZ
Description: Flagellar rod assembly protein/beta-N-acetylglucosaminidase; bifunctional; autolysin
  # bp Upstream # bp Downstream
MW: 34475.16 ---------313 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 1137371
Left Intergenic Region

Name: flgI_flgJ

Length: 1 bp overlap

Orientation: Codirectional+

Left_end: 1137371

Right_end: 1137371

Centisome: 24.50

Genomic Address
Clockwise
Minute or Centisome (%) = 24.50
Right End: 1138312
Right Intergenic Region

Name: flgJ_flgK

Length: 65 bp gap

Orientation: Codirectional+

Left_end: 1138313

Right_end: 1138377

Centisome: 24.52

The C-terminal domain of the orthologous Salmonella enterica FlgJ has endolytic beta-N-acetylglucosaminidase activity, not the previously proposed FlgJ murein hydrolase or lytic transglycosylase activities; the Salmonella active site residues are Glu184 and Glu223, which correspond to Glu181 and Glu220 of E. coli K-12 FlgJ; several errors in Figure 2 are confusing: (1) in 2A Glu225 not Glu223 is boxed, (2) the S. enterica FlgJ numbering from 260 on is one too few in 2A, (3) the misnumbering led to Met300 being mislabeled as Met299 in 2B and to Asp283 being misnumbered in 2A; in addition the PubMed abstract (pmid 25248745) for this paper incorrectly notes Glu233 not Glu223, as an active site residue (Herlihey, 2014). The N-terminal rod-binding domain (Pfam PF10135) of FlgJ is required for rod assembly (Minamino, 1999; Hirano, 2001). The C-terminal domain of FlgJ is required for the autolysin activity that facilitates the rod assembly function of the N-terminal domain but the activity was misinterpreted as a murein hydrolase (Dijkstra, 1996; Nambu, 1999; Hirano, 2001). Flagellar regulon.

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BamHI EcoRI HindIII