GenePage for the uof gene of Escherichia coli K-12

Primary Gene Name: uof
EcoGene Accession Number: EG14465
K-12 Gene Accession Number: ECK4461
MG1655 Gene Identifier: b4637
Gene Name Mnemonic: Upstream Of Fur
Alternate Gene Symbols: None
Description: RyhB-regulated Fur regulatory leader peptide
  # bp Upstream # bp Downstream
MW: 3108.52 ---------28 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 710639
Left Intergenic Region

Name: fur_uof

Length: 8 bp overlap

Orientation: Codirectional-

Left_end: 710639

Right_end: 710646

Centisome: 15.31

Genomic Address
Counterclockwise
Minute or Centisome (%) = 15.31
Right End: 710725
Right Intergenic Region

Name: uof_fldA

Length: 209 bp gap

Orientation: Codirectional-

Left_end: 710726

Right_end: 710934

Centisome: 15.31

The ryhB sRNA is an antisense regulator of uof translation, which is coupled to the downstream fur gene translation; the fur RBS is occluded by 5' mRNA secondary structure, which is opened by uof translation (Vecerek, 2007). A ribosome binding toeprint to the uof translation initiation region was demonstrated, although uof does not have a canonical RBS (Vecerek, 2007).

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BamHI EcoRI HindIII