GenePage for the thiL gene of Escherichia coli K-12

Primary Gene Name: thiL
EcoGene Accession Number: EG20227
K-12 Gene Accession Number: ECK0411
MG1655 Gene Identifier: b0417
Gene Name Mnemonic: Thiamine (and thiazole)
Alternate Gene Symbols: None
Description: Thiamine monophosphate kinase, involved in thiamine salvage
  # bp Upstream # bp Downstream
MW: 35070.85 ---------325 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 435634
Left Intergenic Region

Name: nusB_thiL

Length: 77 bp gap

Orientation: Codirectional+

Left_end: 435557

Right_end: 435633

Centisome: 9.38

Genomic Address
Clockwise
Minute or Centisome (%) = 9.39
Right End: 436611
Right Intergenic Region

Name: thiL_pgpA

Length: 23 bp overlap

Orientation: Codirectional+

Left_end: 436589

Right_end: 436611

Centisome: 9.41

2/12 ThiL peptide masses from MS analysis confirm this start codon and indicate that the initial Met is removed (Maillet, 2007). ThiL peptide masses from MS/MS analysis indicate this is the correct start site and that the initial Met is removed (Guo, 2014). otherPDB (S. typhimurium): 1VQV.

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BamHI EcoRI HindIII