GenePage for the insN' pseudogene of Escherichia coli K-12

Primary Gene Name: insN'
EcoGene Accession Number: EG40014
K-12 Gene Accession Number: ECK0257
MG1655 Gene Identifier: b4587, b0257, b0255
Gene Name Mnemonic: Insertion Sequence
Alternate Gene Symbols: insN1; insO; ORFAB
Description: Pseudogene reconstruction, fused IS911 transposase AB
  # bp Upstream # bp Downstream
Verified Start MW: 26057.03 ---------229 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 270278
Left Intergenic Region

Name: perR_insN

Length: 95 bp gap

Orientation: Divergent

Left_end: 270183

Right_end: 270277

Centisome: 5.82

Genomic Address join

The following join statement uses GenBank syntax to describe how the reconstructed insN pseudogene protein sequence was translated from the DNA:

join(270278..270535,271764..272189)


Clockwise
Minute or Centisome (%) = 5.82
Right End: 272189
Right Intergenic Region

Name: insN_insI1

Length: 67 bp gap

Orientation: Codirectional+

Left_end: 270536

Right_end: 270602

Centisome: 5.83

The individual ORFA and ORFB proteins are also observed, but the fused ORFAB protein is the active transposase. Intact alleles of ORFAB include Q6Q6S9 and Q8GJ19. insN is interrupted by the insertion of IS30A at codon 86, also the site of translation frameshifting in the intact ORFAB. Two aminos acids are deleted at the insertion site (G87, Y88), represented by XX. The C-terminus is truncated at the site of ISY insertion, leaving 229 N-terminal residues. The annotated start site was verified for a wildtype IS911.

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BamHI EcoRI HindIII