EcoGene

GenePage for the dnaK gene of Escherichia coli K-12

Primary Gene Name: dnaK
EcoGene Accession Number: EG10241
K-12 Gene Accession Number: ECK0014
MG1655 Gene Identifier: b0014

APEX

Biofilm Defective Mutants

Common Core

CommonCore-5

Curved Promoters

Extracellular Proteome

Heat-stable Proteins

[Pt(dien)(H2O)]2+

Gene Name Mnemonic: DNA

Alternate Gene Symbols: groPAB, groPC, groPF, grpC, grpF, seg

Description:
Hsp70 molecular chaperone, heat-inducible; bichaperone with ClpB for protein disaggregation

Pre-Run BlastP UniProt

Protein Sequence: ------------ MW: 69114.96------638 aa ------------ Pre-Run BlastP NR+Env

Genomic Address
Left End: 12163 ----------------- Clockwise ----------------- Right End: 14079
Left Inter Gene Info Minute or Centisome (%) = 0.26 Right Inter Gene Info

PDF EcoMap

Curator or user-submitted Comments:

ClpB and DnaK cooperate (with DnaJ and GrpE) in the bichaperone-mediated, ATP-dependent unfolding of protein aggregates (Zolkiewski, 1999; Goloubinoff, 1999). ATP-regulated binding and release of polypeptide substrates. Binds Zn(II). DnaJ is co-chaperone. Role in the initiation of DNA replication. DnaK has secondary amide peptide bond cis-trans isomerase (APIase) activity. A dnaK mutant is inviable above 42C, or below 8C (as enabled by transgenic Oleispira antarctica Cpn10/60).  tig dnaK double deletionis a synthetic lethal combination at 30 degrees C, however, no lethal effects are observed at 23 degrees C; overproduction of GroEL/GroES at 30 degrees C supresses synthetic lethality. dnaK, hscA, hscC and yegD are paralogs. HT_Cmplx22_Cyt: DnaK+GrpE+GyrA+RpsA. HT_Cmplx30_Mem: ClpB+DnaK+InfB+RpsA. After four hours of Zn(II) stress, protein levels increased (Easton, 2006).