EcoGene

GenePage for the ileS gene of Escherichia coli K-12

Primary Gene Name: ileS
EcoGene Accession Number: EG10492
K-12 Gene Accession Number: ECK0027
MG1655 Gene Identifier: b0026

Gene Name Mnemonic: Isoleucine

Alternate Gene Symbols: ilvS

Description:
Isoleucine--tRNA ligase

Pre-Run BlastP UniProt

Protein Sequence: ------------ MW: 104296.84------938 aa ------------ Pre-Run BlastP NR+Env

Genomic Address
Left End: 22391 ----------------- Clockwise ----------------- Right End: 25207
Left Inter Gene Info Minute or Centisome (%) = 0.48 Right Inter Gene Info

PDF EcoMap

Curator or user-submitted Comments:

The entire protein seqeunce of IleS has been determined using peptide mapping by MALDI mass spectrometry; 9 aa differences were found as compared to the current DNA-derived protein sequence (300-EL>DV-301; 724G>V;738-AKADSV>PKRTVW-743); whereas the minor differences could be strain polymorphisms, the 6 aa difference may indicate two (in-and-out) closely-spaced DNA sequencing frameshift errors (Baouz, 2009). ileS is an essential gene; the ileS mutants in the Keio collection have a second intact copy of ileS (R. D'Ari and K. Nakahihashi, personal communication cited in Baba, 2000).