EcoGene

Stoebel,2009 All Experiments Top

gene experimental details:
Exp ID Exp Data Gene name Allele Comments
3 Show Data rpoS+2 WT evolved strain rpoS+2

Kohanski,2007 All Experiments Top

chemical experimental details:
Exp ID Exp Data Name Concentration Time added Duration Comments
1 Show Data norfloxacin (30min) 250 ng/ml early log phase 30 min
3 Show Data norfloxacin (120min) 250 ng/ml early log phase 120 min
11 Show Data norfloxacin (60min) / spectinmycin 250 ng/ml early log phase 60 min
13 Show Data ampicillin (30min) / spectinmycin 5 ug/ml early log phase 30 min
16 Show Data kanamycin (30min) / spectinmycin 5 ug/ml early log phase 30 min

Kabir,2005 All Experiments Top

gene experimental details:
Exp ID Exp Data Gene name Allele Comments
2 Show Data rpoE over expression Arabinose was added at 12hr (early stationary phase), RNA was extracted 1hr post induction. See paper comment for important data information.
1 Show Data rpoE over expression Arabinose was added at 6hr (mid exponential phase), RNA was extracted 1hr post induction. See paper comment for important data information.

Shimizu,2005 All Experiments Top

chemical experimental details:
Exp ID Exp Data Name Concentration Time added Duration Comments
5 Show Data cyclohexane:p-xylene (6:4 (v/v)) 10% (v/v) initially 10 min TK31 is a spontaneous p-xylene-tolerant mutant derived from JA300, LBGMg media

Peter,2004 All Experiments Top

chemical experimental details:
Exp ID Exp Data Name Concentration Time added Duration Comments
7 Show Data norfloxacin 50ug/ml 5 min gyrAr/parCr cells, publication indicates no changes in supercoiling, transcription profile changes due to unknown targets of antibiotic.
8 Show Data norfloxacin 50ug/ml 10 min gyrAr/parCCr cells, publication indicates no changes in supercoiling, transcription profile changes due to unknown targets of antibiotic.
9 Show Data norfloxacin 50ug/ml 20 min gyrAr/parCCr cells, publication indicates no changes in supercoiling, transcription profile changes due to unknown targets of antibiotic.
10 Show Data norfloxacin 50ug/ml 30min gyrAr/parCCr cells, publication indicates no changes in supercoiling, transcription profile changes due to unknown targets of antibiotic.

Li,2003 All Experiments Top

gene experimental details:
Exp ID Exp Data Gene name Allele Comments
1 Show Data rnpA A49 to G(temperature sensitive, point mutation) At OD600=0.25 culture was shifted from 30C to 42C. 0min time point.
2 Show Data rnpA A49 to G(temperature sensitive) RNA was extracted 15 min post shift from 30C to 42C.

Sabina,2003 All Experiments Top

chemical experimental details:
Exp ID Exp Data Name Concentration Time added Duration Comments
3 Show Data kasugamycin 750 ug/ml OD=0.4 30 min M9+0.4% glucose; reagent was added at OD600=0.4 and RNA was extracted 30min post addition.

Gutiérrez-Ríos,2003 All Experiments Top

growth experimental details:
Exp ID Exp Data Name Variable Shift time Duration Comments
1 Show Data 37C vs 50C 50C mid log 5 min cells were grown in minimal MOPS+0.2% glucose, shifted from 37C to 50C; RNA was extracted 5 min post shift.
2 Show Data mid log vs stationary stationary steady OD600=1.5 cells were grown to stationary phase in MOPS+ 0.2% glucose.