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  Microarray Experiment Search Results for sgrR
25 total
Reference Variable Value    Experiments Contact
Kohanski,2007  norfloxacin (30min) / spectinmycin -2.53 Chemical_10, Exp Data James J. Collins
Kohanski,2007  norfloxacin (60min) / spectinmycin 2.171 Chemical_11, Exp Data James J. Collins
Kohanski,2007  kanamycin (60min) / spectinmycin 2.218 Chemical_17, Exp Data James J. Collins
Gadgil,2005  37C vs 28C 2.713 Growth_9, Exp Data Wei-Shou Hu
Gadgil,2005  37C vs 33C 2.732 Growth_2, Exp Data Wei-Shou Hu
Gadgil,2005  37C vs 28C 3.182 Growth_10, Exp Data Wei-Shou Hu
Gadgil,2005  37C vs 33C 4.141 Growth_3, Exp Data Wei-Shou Hu
Gadgil,2005  37C vs 28C 12.641 Growth_11, Exp Data Wei-Shou Hu
Gadgil,2005  37C vs 33C 3.458 Growth_4, Exp Data Wei-Shou Hu
Gadgil,2005  37C vs 33C 2.549 Growth_5, Exp Data Wei-Shou Hu
Gadgil,2005  37C vs 28C 2.694 Growth_14, Exp Data Wei-Shou Hu
Yoshida,2004  putrescine -6.667 Chemical_1, Exp Data Kazuei Igarashi
Peter,2004  norfloxacin 2.17 Chemical_13, Exp Data Brian J Peter
Peter,2004  norfloxacin  2.149 Chemical_7, Exp Data Brian J Peter
Peter,2004  norfloxacin 2.43 Chemical_9, Exp Data Brian J Peter
Peter,2004  novobiocin 2.341 Chemical_25, Exp Data Brian J Peter
Peter,2004  novobiocin  3.66 Chemical_26, Exp Data Brian J Peter
Peter,2004  novobiocin  2.951 Chemical_27, Exp Data Brian J Peter
Peter,2004  37C vs 42C 2.28 Growth_28, Exp Data Brian J Peter
Peter,2004  37C vs 42C 3.149 Growth_29, Exp Data Brian J Peter
Peter,2004  gyrB  3.85 Gene_32, Exp Data Brian J Peter
Peter,2004  gyrB  5.039 Gene_33, Exp Data Brian J Peter
Peter,2004  gyrB  3.2 Gene_34, Exp Data Brian J Peter
Li,2003  rnpA  2 Gene_2, Exp Data Sidney Altman
Li,2003  rnpA  2.6 Gene_4, Exp Data Sidney Altman


Experimental Details

Kohanski,2007 All Experiments Top

chemical experimental details:
Exp ID Exp Data Name Concentration Time added Duration Comments
10Show Datanorfloxacin (30min) / spectinmycin250 ng/mlearly log phase30 min
11Show Datanorfloxacin (60min) / spectinmycin250 ng/mlearly log phase60 min
17Show Datakanamycin (60min) / spectinmycin5 ug/mlearly log phase60 min




Gadgil,2005 All Experiments Top

growth experimental details:
Exp ID Exp Data Name Variable Shift time Duration Comments
9Show Data37C vs 28C28COD600=0.610 minCells were shifted from 37C to 28C, RNA was extracted 10 min post shift. Cultures were grown in minimal media.
2Show Data37C vs 33C33COD600=0.610 minCells were shifted from 37C to 33C, RNA was extracted 10 min post shift. Cultures were grown in minimal media.
10Show Data37C vs 28C28COD600=0.620 minCells were shifted from 37C to 28C, RNA was extracted 20 min post shift. Cultures were grown in minimal media.
3Show Data37C vs 33C33COD600=0.620 minCells were shifted from 37C to 33C, RNA was extracted 20 min post shift. Cultures were grown in minimal media.
11Show Data37C vs 28C28COD600=0.630 minCells were shifted from 37C to 28C, RNA was extracted 30 min post shift. Cultures were grown in minimal media.
4Show Data37C vs 33C33COD600=0.630 minCells were shifted from 37C to 33C, RNA was extracted 30 min post shift. Cultures were grown in minimal media.
5Show Data37C vs 33C33COD600=0.660 minCells were shifted from 37C to 33C, RNA was extracted 60 min post shift. Cultures were grown in minimal media.
14Show Data37C vs 28C28COD600=0.6120 minCells were shifted from 37C to 28C, RNA was extracted 120 min post shift. Cultures were grown in minimal media.




Yoshida,2004 All Experiments Top

chemical experimental details:
Exp ID Exp Data Name Concentration Time added Duration Comments
1Show Dataputrescine100 µg/mlA540=0.2Medium A + 5 100mM of each of the following amino acids: gly, leu, met, ser, thr; MA261 requires polyamines.




Peter,2004 All Experiments Top

chemical experimental details:
Exp ID Exp Data Name Concentration Time added Duration Comments
13Show Datanorfloxacin15 ug/ml10 mingyrA+/parCr
7Show Datanorfloxacin 50ug/ml5 mingyrAr/parCr cells, publication indicates no changes in supercoiling, transcription profile changes due to unknown targets of antibiotic.
9Show Datanorfloxacin50ug/ml20 mingyrAr/parCCr cells, publication indicates no changes in supercoiling, transcription profile changes due to unknown targets of antibiotic.
25Show Datanovobiocin20 ug/ml5 min
26Show Datanovobiocin 50 ug/ml5 min
27Show Datanovobiocin 200 ug/ml5 min

growth experimental details:
Exp ID Exp Data Name Variable Shift time Duration Comments
28Show Data37C vs 42C42Csteady2 mingyrB+, served as a control for the gyrB ts experiments to ensure changes due to gyrB mutation, not temperature shift.
29Show Data37C vs 42C42Csteady5 mingyrB+, served as a control for the gyrB ts experiments to ensure changes due to gyrB mutation, not temperature shift.

gene experimental details:
Exp ID Exp Data Gene name Allele Comments
32Show DatagyrBgyrB ts(temperature sensitive)42C for 2 minutes compared to gyrBts at 37C
33Show DatagyrBgyrB ts(temperature sensitive)42C for 5 minutes compared to gyrB ts at 37C
34Show DatagyrBgyrB ts(temperature sensitive)42C for 10 minutes compared to gyrB ts at 37C




Li,2003 All Experiments Top

gene experimental details:
Exp ID Exp Data Gene name Allele Comments
2Show DatarnpAA49 to G(temperature sensitive)RNA was extracted 15 min post shift from 30C to 42C.
4Show DatarnpAA49 to G(temperature sensitive)RNA extracted at 15 min post 42C shift was compared to RNA extracted at 45 min post 42C shift.








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