Microarray Experiment Search Results for
sgrR
25 total
Reference
Variable
Value
fold
ratio
ln
log2
Experiments
Contact
Kohanski,2007
norfloxacin (30min) / spectinmycin
-2.53
Chemical_10
,
Exp Data
James J. Collins
Kohanski,2007
norfloxacin (60min) / spectinmycin
2.171
Chemical_11
,
Exp Data
James J. Collins
Kohanski,2007
kanamycin (60min) / spectinmycin
2.218
Chemical_17
,
Exp Data
James J. Collins
Gadgil,2005
37C vs 28C
2.713
Growth_9
,
Exp Data
Wei-Shou Hu
Gadgil,2005
37C vs 33C
2.732
Growth_2
,
Exp Data
Wei-Shou Hu
Gadgil,2005
37C vs 28C
3.182
Growth_10
,
Exp Data
Wei-Shou Hu
Gadgil,2005
37C vs 33C
4.141
Growth_3
,
Exp Data
Wei-Shou Hu
Gadgil,2005
37C vs 28C
12.641
Growth_11
,
Exp Data
Wei-Shou Hu
Gadgil,2005
37C vs 33C
3.458
Growth_4
,
Exp Data
Wei-Shou Hu
Gadgil,2005
37C vs 33C
2.549
Growth_5
,
Exp Data
Wei-Shou Hu
Gadgil,2005
37C vs 28C
2.694
Growth_14
,
Exp Data
Wei-Shou Hu
Yoshida,2004
putrescine
-6.667
Chemical_1
,
Exp Data
Kazuei Igarashi
Peter,2004
norfloxacin
2.17
Chemical_13
,
Exp Data
Brian J Peter
Peter,2004
norfloxacin
2.149
Chemical_7
,
Exp Data
Brian J Peter
Peter,2004
norfloxacin
2.43
Chemical_9
,
Exp Data
Brian J Peter
Peter,2004
novobiocin
2.341
Chemical_25
,
Exp Data
Brian J Peter
Peter,2004
novobiocin
3.66
Chemical_26
,
Exp Data
Brian J Peter
Peter,2004
novobiocin
2.951
Chemical_27
,
Exp Data
Brian J Peter
Peter,2004
37C vs 42C
2.28
Growth_28
,
Exp Data
Brian J Peter
Peter,2004
37C vs 42C
3.149
Growth_29
,
Exp Data
Brian J Peter
Peter,2004
gyrB
3.85
Gene_32
,
Exp Data
Brian J Peter
Peter,2004
gyrB
5.039
Gene_33
,
Exp Data
Brian J Peter
Peter,2004
gyrB
3.2
Gene_34
,
Exp Data
Brian J Peter
Li,2003
rnpA
2
Gene_2
,
Exp Data
Sidney Altman
Li,2003
rnpA
2.6
Gene_4
,
Exp Data
Sidney Altman
Experimental Details
Kohanski,2007
All Experiments
Top
chemical experimental details:
Exp ID
Exp Data
Name
Concentration
Time added
Duration
Comments
10
Show Data
norfloxacin (30min) / spectinmycin
250 ng/ml
early log phase
30 min
11
Show Data
norfloxacin (60min) / spectinmycin
250 ng/ml
early log phase
60 min
17
Show Data
kanamycin (60min) / spectinmycin
5 ug/ml
early log phase
60 min
Gadgil,2005
All Experiments
Top
growth experimental details:
Exp ID
Exp Data
Name
Variable
Shift time
Duration
Comments
9
Show Data
37C vs 28C
28C
OD600=0.6
10 min
Cells were shifted from 37C to 28C, RNA was extracted 10 min post shift. Cultures were grown in minimal media.
2
Show Data
37C vs 33C
33C
OD600=0.6
10 min
Cells were shifted from 37C to 33C, RNA was extracted 10 min post shift. Cultures were grown in minimal media.
10
Show Data
37C vs 28C
28C
OD600=0.6
20 min
Cells were shifted from 37C to 28C, RNA was extracted 20 min post shift. Cultures were grown in minimal media.
3
Show Data
37C vs 33C
33C
OD600=0.6
20 min
Cells were shifted from 37C to 33C, RNA was extracted 20 min post shift. Cultures were grown in minimal media.
11
Show Data
37C vs 28C
28C
OD600=0.6
30 min
Cells were shifted from 37C to 28C, RNA was extracted 30 min post shift. Cultures were grown in minimal media.
4
Show Data
37C vs 33C
33C
OD600=0.6
30 min
Cells were shifted from 37C to 33C, RNA was extracted 30 min post shift. Cultures were grown in minimal media.
5
Show Data
37C vs 33C
33C
OD600=0.6
60 min
Cells were shifted from 37C to 33C, RNA was extracted 60 min post shift. Cultures were grown in minimal media.
14
Show Data
37C vs 28C
28C
OD600=0.6
120 min
Cells were shifted from 37C to 28C, RNA was extracted 120 min post shift. Cultures were grown in minimal media.
Yoshida,2004
All Experiments
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chemical experimental details:
Exp ID
Exp Data
Name
Concentration
Time added
Duration
Comments
1
Show Data
putrescine
100 µg/ml
A540=0.2
Medium A + 5 100mM of each of the following amino acids: gly, leu, met, ser, thr; MA261 requires polyamines.
Peter,2004
All Experiments
Top
chemical experimental details:
Exp ID
Exp Data
Name
Concentration
Time added
Duration
Comments
13
Show Data
norfloxacin
15 ug/ml
10 min
gyrA+/parCr
7
Show Data
norfloxacin
50ug/ml
5 min
gyrAr/parCr cells, publication indicates no changes in supercoiling, transcription profile changes due to unknown targets of antibiotic.
9
Show Data
norfloxacin
50ug/ml
20 min
gyrAr/parCCr cells, publication indicates no changes in supercoiling, transcription profile changes due to unknown targets of antibiotic.
25
Show Data
novobiocin
20 ug/ml
5 min
26
Show Data
novobiocin
50 ug/ml
5 min
27
Show Data
novobiocin
200 ug/ml
5 min
growth experimental details:
Exp ID
Exp Data
Name
Variable
Shift time
Duration
Comments
28
Show Data
37C vs 42C
42C
steady
2 min
gyrB+, served as a control for the gyrB ts experiments to ensure changes due to gyrB mutation, not temperature shift.
29
Show Data
37C vs 42C
42C
steady
5 min
gyrB+, served as a control for the gyrB ts experiments to ensure changes due to gyrB mutation, not temperature shift.
gene experimental details:
Exp ID
Exp Data
Gene name
Allele
Comments
32
Show Data
gyrB
gyrB ts(temperature sensitive)
42C for 2 minutes compared to gyrBts at 37C
33
Show Data
gyrB
gyrB ts(temperature sensitive)
42C for 5 minutes compared to gyrB ts at 37C
34
Show Data
gyrB
gyrB ts(temperature sensitive)
42C for 10 minutes compared to gyrB ts at 37C
Li,2003
All Experiments
Top
gene experimental details:
Exp ID
Exp Data
Gene name
Allele
Comments
2
Show Data
rnpA
A49 to G(temperature sensitive)
RNA was extracted 15 min post shift from 30C to 42C.
4
Show Data
rnpA
A49 to G(temperature sensitive)
RNA extracted at 15 min post 42C shift was compared to RNA extracted at 45 min post 42C shift.
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