EcoGene

GenePage for the dnaK gene of Escherichia coli K-12

ChemGen Gene Correlations: dnaK

ChemGen Growth Data: dnaK

Gene-Profiles.org: dnaK

Genevestigator: P0A6Y8

GenExpDB: b0014

GenoBaseMG: b0014

Genome3D: P0A6Y8

GenomeReviews: P0A6Y8

GenPept-GenBank: AAC73125

GenPept-RefSeq: NP_414555.1

Inparanoid: NP_414555.1

iProClass: P0A6Y8

iRefWeb: 944750

Membranome: DNAK_ECOLI

MicrobesOnline: P0A6Y8

ProteinOntology: 000022465

ProteinOntology: 000024933

ProteomeFolding at YRC: P0A6Y8

ProtMap_COG: COG0443#NC_000913

ProtoNet: P0A6Y8

QUARK: E11336

QuickGO: P0A6Y8

RegTransBase: 8798426

STRING: b0014

SWISS-2D: P0A6Y8

TCDB: P0A6Y8&tc=1.A.33.1.2

TCDB_Fam: 1.A.33

TF Binding Sites: dnaK.html

TIGR-CMR: NT01EC0015

Transimulation: b0014

Unison: P0A6Y8

wikigenes: 944750

WolframAlpha: dnaK

xbase-ColiBASE: b0014

Primary Gene Name: dnaK
EcoGene Accession Number: EG10241
K-12 Gene Accession Number: ECK0014
MG1655 Gene Identifier: b0014

APEX

AUG Start Codons

Biofilm Defective Mutants

Extracellular Proteome

Heat-stable Proteins

MIRAGE-Zn

Protein Lysine Acetylation: Yu, 2008

Total Proteome Lopez2005

Verified Set

Verified Translation Starts (+Met)

Verified Translation Starts Sites

[Pt(dien)(H2O)]2+

Gene Name Mnemonic: DNA

Alternate Gene Symbols: groPAB, groPC, groPF, grpC, grpF, seg

Description:
Hsp70 molecular chaperone, heat-inducible; bichaperone with ClpB for protein disaggregation

Pre-Run BlastP UniProt

Protein Sequence ------------ MW: 69114.96------638 aa ------------ Pre-Run BlastP NR+Env

Genomic Address
Left End: 12163 ----------------- Clockwise ----------------- Right End: 14079
Left Inter Gene Info Minute or Centisome (%) = 0.26 Right Inter Gene Info

PDF EcoMap

Curator or user-submitted Comments:

ClpB and DnaK cooperate (with DnaJ and GrpE) in the bichaperone-mediated, ATP-dependent unfolding of protein aggregates (Zolkiewski, 1999; Goloubinoff, 1999). DnaK mediates ATP-regulated binding and release of polypeptide substrates. Binds Zn(II). DnaJ is a co-chaperone for DnaK. Role in the initiation of DNA replication. DnaK has secondary amide peptide bond cis-trans isomerase (APIase) activity. A dnaK mutant is inviable above 42C, or below 8C (as enabled by transgenic Oleispira antarctica Cpn10/60). A tig dnaK double deletion is a synthetic lethal combination at 30 degrees C, however, no lethal effects are observed at 23 degrees C; overproduction of GroEL/GroES at 30 degrees C suppresses synthetic lethality. dnaK, hscA, hscC and yegD are paralogs. After four hours of Zn(II) stress, protein levels increased (Easton, 2006). DnaK lysines K70, K245, K246, K304, K359, K502, K528 and K587 can be modified to N6-succinyllysine (Zhang, 2011). DnaK lysines K109, K245, K304, K421, K556 and K577 can be modified to N6-acetyllysine (Yu, 2008; Zhang, 2009). HT_Cmplx22_Cyt: DnaK+GrpE+GyrA+RpsA. HT_Cmplx30_Mem: ClpB+DnaK+InfB+RpsA.