EcoGene

GenePage for the rpsL gene of Escherichia coli K-12

Bacteriome: rpsL

ChemGen Gene Correlations: rpsL

ChemGen Growth Data: rpsL

Gene-Profiles.org: rpsL

Genevestigator: P0A7S3

GenExpDB: b3342

Genome3D: P0A7S3

GenomeReviews: P0A7S3

GenPept: NP_417801.1

GenPept-GenBank: AAC76367

MicrobesOnline: P0A7S3

ProteinOntology: 000023865

ProteinOntology: 000024995

ProteomeFolding at YRC: P0A7S3

ProtoNet: P0A7S3

QuickGO: P0A7S3

RegulonDB-TU: ECK120014637

STRING: P0A7S3

SWISS-2D: P0A7S3

TF Binding Sites: rpsL.html

xbase-ColiBASE: b3342

Primary Gene Name: rpsL
EcoGene Accession Number: EG10911
K-12 Gene Accession Number: ECK3329
MG1655 Gene Identifier: b3342

Small Ribosomal Proteins

Verified Set

Verified Translation Starts (+Met)

Verified Translation Starts Sites

Gene Name Mnemonic: Ribosomal protein, small

Alternate Gene Symbols: asuB, strA

Description:
30S ribosomal subunit protein S12; RNA chaperone

Pre-Run BlastP UniProt

Protein Sequence ------------ MW: 13737.06------124 aa ------------ Pre-Run BlastP NR+Env

Genomic Address
Left End: 3472200 ----------------- Counterclockwise ----------------- Right End: 3472574
Left Inter Gene Info Minute or Centisome (%) = 74.84 Right Inter Gene Info

PDF EcoMap

Curator or user-submitted Comments:

RimO catalyzes the methylthiolation of Asp-89 of ribosomal protein S12 to form 3-methylthioaspartic acid (Kowalak, 1996; Anton, 2008). YcaO is required for the efficient in vivo RimO-dependent beta-methylthiolation of ribosomal protein S12 (RpsL) on Asp-89 (Strader, 2011). Asp-88 of the mature RpsL is Asp-89 of the unmodified protein. RpsL lysine K108 can be modifed to N6-acetyllysine (Zhang, 2009). Mutations in regions 41-45 (except when Lys42 is replaced with Arg), 87-93 and 53 result in streptomycin resistance. A point mutation at basepair 262 from A to G sustains protein synthesis in late stationary phase and increases the stability of the 70S complex in low magnesium concentrations.