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TopicPage for MemProtEx-Plus of Escherichia coli K-12

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MemProtExpress         
        

Description:

Savage (2007) targetted 120 E. coli membrane proteins for in vivo and in vitro expression.
These 91 MemProtEx-Plus proteins were expressed at some level.

Six proteins were expressed at strong levels both in vivo and in vitro:
DgkA, MscL, PgpA, YgaP, YidH, and YjeO.

Five proteins thst were expressed well in the cell-free S30 system were affinity purified on nickel columns:
DgkA, PsiE, YidG, YijD, and YiaA.

Details:

Savage et al. (2007) testing expression of 117 N-terminal His-tagged clones in vivo using a pET3-derived vector in BL21(DE3) cells.
In vivo expression, and in vitro expression using S30 extracts, were assessed as negative, weak positive, or strong postive using Western blots.

33 MemProtEx-Both proteins were expressed both in vitro and in vivo.
18 MemProtEx-VivoOnly proteins were expressed only in vivo.
40 MemProtEx-VitroOnly proteins were expressed only in vitro.

15 MemProtEx-VivoStrong proteins were expressed strongly in vivo ( > 2 mg/L).
35 MemProtEx-VitroStrong proteins were expressed strongly in vitro ( > 0.2 mg/ml).

36 MemProtEx-VivoWeak proteins were expressed weakly in vivo ( < 2 mg/L).
38 MemProtEx-VitroWeak proteins were expressed weakly in vitro ( < 2 mg/L).


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