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TopicPage for MemProtEx-VivoStrong of Escherichia coli K-12

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Savage (2007) targetted 120 E. coli membrane proteins for in vivo and in vitro expression.
These 15 MemProtEx-VivoStrong proteins were expressed strongly in vivo ( > 2 mg/L).


Savage et al. (2007) testing expression of 117 N-terminal His-tagged clones in vivo using a pET3-derived vector in BL21(DE3) cells.
In vivo expression, and in vitro expression using S30 extracts, were assessed as negative, weak positive, or strong postive using Western blots.

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