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TopicPage for MemProtEx-VitroWeak of Escherichia coli K-12

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MemProtEx-Plus         

Description:

Savage (2007) targetted 120 E. coli membrane proteins for in vivo and in vitro expression.
These 38 MemProtEx-VitroWeak proteins were expressed weakly in vitro ( < 2 mg/L).

Details:

Savage et al. (2007) testing expression of 117 N-terminal His-tagged clones in vivo using a pET3-derived vector in BL21(DE3) cells.
In vivo expression, and in vitro expression using S30 extracts, were assessed as negative, weak positive, or strong postive using Western blots.




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