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EcogeneTopic Page
TopicPage for DNA Replication of Escherichia coli K-12
 | Chromosome Metabolism          |
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Description:
Bidirectional DNA replication forks of the E. coli chromosome (2) initiate at oriC, (2) elongate convergently on sister chromophores, (3) assist in repair and recombination functions, (4) occassionally stall, (5) cross unidirection pause sites Ter A-F, and finally terminate at the intergene dif locus, where daughter chromsomes decatenate and separate.
Details:
At fast growth rates, multiple forks can be replicating the same DNA molecule simultaneously, in staged pairs.
The terminus region is dispensible and E. coli can replicate its chromsome as a linear molecule (Cui, 2007).
Bibliography (57 total) : Review Only   Up
- 2013
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Corzett CH, Goodman MF, Finkel SE (2013) Competitive Fitness During Feast and Famine: How SOS DNA Polymerases Influence Physiology and Evolution in Escherichia coli. Genetics 194:409-20
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Gupta MK, Guy CP, Yeeles JT, Atkinson J, Bell H, Lloyd RG, Marians KJ, McGlynn P (2013) Protein-DNA complexes are the primary sources of replication fork pausing in Escherichia coli. Proc Natl Acad Sci U S A 110:7252-7
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Kurth I, Georgescu RE, O'Donnell ME (2013) A solution to release twisted DNA during chromosome replication by coupled DNA polymerases. Nature 496:119-22
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Indiani C, Patel M, Goodman MF, O'Donnell ME (2013) RecA acts as a switch to regulate polymerase occupancy in a moving replication fork. Proc Natl Acad Sci U S A 110:5410-5
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Skarstad K, Katayama T (2013) Regulating DNA replication in bacteria. Cold Spring Harb Perspect Biol 5:a012922
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Lia G, Rigato A, Long E, Chagneau C, Le Masson M, Allemand JF, Michel B (2013) RecA-promoted, RecFOR-independent progressive disassembly of replisomes stalled by helicase inactivation. Mol Cell 49:547-57
- 2012
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Fijalkowska IJ, Schaaper RM, Jonczyk P (2012) DNA replication fidelity in Escherichia coli: a multi-DNA polymerase affair. FEMS Microbiol Rev 36:1105-21 Review
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Pagès V, Mazon G, Naiman K, Philippin G, Fuchs RP (2012) Monitoring bypass of single replication-blocking lesions by damage avoidance in the Escherichia coli chromosome. Nucleic Acids Res 40:9036-43
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Lia G, Michel B, Allemand JF (2012) Polymerase exchange during Okazaki fragment synthesis observed in living cells. Science 335:328-31
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De Septenville AL, Duigou S, Boubakri H, Michel B (2012) Replication fork reversal after replication-transcription collision. PLoS Genet 8:e1002622
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Hill NS, Kadoya R, Chattoraj DK, Levin PA (2012) Cell size and the initiation of DNA replication in bacteria. PLoS Genet 8:e1002549
- 2011
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Yeeles JT, Marians KJ (2011) The Escherichia coli replisome is inherently DNA damage tolerant. Science 334:235-8
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Gan W, Guan Z, Liu J, Gui T, Shen K, Manley JL, Li X (2011) R-loop-mediated genomic instability is caused by impairment of replication fork progression. Genes Dev 25:2041-56
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Kaguni JM (2011) Replication initiation at the Escherichia coli chromosomal origin. Curr Opin Chem Biol 15:606-13
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Dutta D, Shatalin K, Epshtein V, Gottesman ME, Nudler E (2011) Linking RNA polymerase backtracking to genome instability in E. coli. Cell 146:533-43
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Wang X, Lesterlin C, Reyes-Lamothe R, Ball G, Sherratt DJ (2011) Replication and segregation of an Escherichia coli chromosome with two replication origins. Proc Natl Acad Sci U S A 108:E243-50
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Tanner NA, Tolun G, Loparo JJ, Jergic S, Griffith JD, Dixon NE, van Oijen AM (2011) E. coli DNA replication in the absence of free ? clamps. EMBO J 30:1830-40
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Norris V (2011) Speculations on the initiation of chromosome replication in Escherichia coli: the dualism hypothesis. Med Hypotheses 76:706-16
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Stahl F, Meselson M (2011) Letter to the editor. DNA Repair (Amst) 10:118 Comment
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Le HT, Gautier V, Kthiri F, Kohiyama M, Katayama T, Richarme G (2011) DNA replication defects in a mutant deficient in the thioredoxin homolog YbbN. Biochem Biophys Res Commun 405:52-7
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Martín CM, Guzmán EC (2011) DNA replication initiation as a key element in thymineless death. DNA Repair (Amst) 10:94-101
- 2010
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Pomerantz RT, O'Donnell M (2010) Polymerase trafficking: A role for transcription factors in preventing replication fork arrest. Transcription 1:136-139
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Ton-Hoang B, Pasternak C, Siguier P, Guynet C, Hickman AB, Dyda F, Sommer S, Chandler M (2010) Single-stranded DNA transposition is coupled to host replication. Cell 142:398-408
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Pomerantz RT, O'Donnell M (2010) What happens when replication and transcription complexes collide? Cell Cycle 9:2537-43 Review
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Georgescu RE, Yao NY, O'Donnell M (2010) Single-molecule analysis of the Escherichia coli replisome and use of clamps to bypass replication barriers. FEBS Lett 584:2596-605 Review
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Yao NY, O'Donnell M (2010) SnapShot: The replisome. Cell 141:1088, 1088.e1
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Liu X, Wang X, Reyes-Lamothe R, Sherratt D (2010) Replication-directed sister chromosome alignment in Escherichia coli. Mol Microbiol 75:1090-7
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Katayama T, Ozaki S, Keyamura K, Fujimitsu K (2010) Regulation of the replication cycle: conserved and diverse regulatory systems for DnaA and oriC. Nat Rev Microbiol 8:163-70
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Boubakri H, de Septenville AL, Viguera E, Michel B (2010) The helicases DinG, Rep and UvrD cooperate to promote replication across transcription units in vivo. EMBO J 29:145-57
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Jeiranian HA, Schalow BJ, Courcelle J (2010) Visualization of UV-induced replication intermediates in E. coli using two-dimensional agarose-gel analysis. J Vis Exp :
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Pomerantz RT, O'Donnell M (2010) Direct restart of a replication fork stalled by a head-on RNA polymerase. J Vis Exp :
- 2009
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Christian TD, Romano LJ, Rueda D (2009) Single-molecule measurements of synthesis by DNA polymerase with base-pair resolution. Proc Natl Acad Sci U S A 106:21109-14
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Langston LD, Indiani C, O'Donnell M (2009) Whither the replisome: emerging perspectives on the dynamic nature of the DNA replication machinery. Cell Cycle 8:2686-91 Review
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Yao NY, Georgescu RE, Finkelstein J, O'Donnell ME (2009) Single-molecule analysis reveals that the lagging strand increases replisome processivity but slows replication fork progression. Proc Natl Acad Sci U S A 106:13236-41
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Flåtten I, Morigen , Skarstad K (2009) DnaA protein interacts with RNA polymerase and partially protects it from the effect of rifampicin. Mol Microbiol 71:1018-30
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Odsbu I, Morigen , Skarstad K (2009) A reduction in ribonucleotide reductase activity slows down the chromosome replication fork but does not change its localization. PLoS One 4:e7617
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Tanner NA, van Oijen AM (2009) Single-molecule observation of prokaryotic DNA replication. Methods Mol Biol 521:397-410
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Dalgaard JZ, Eydmann T, Koulintchenko M, Sayrac S, Vengrova S, Yamada-Inagawa T (2009) Random and site-specific replication termination. Methods Mol Biol 521:35-53 Review
- 2008
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Pomerantz RT, O'Donnell M (2008) The replisome uses mRNA as a primer after colliding with RNA polymerase. Nature 456:762-6
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Duggin IG, Wake RG, Bell SD, Hill TM (2008) The replication fork trap and termination of chromosome replication. Mol Microbiol 70:1323-33 Review
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Yao NY, O'Donnell M (2008) Replisome dynamics and use of DNA trombone loops to bypass replication blocks. Mol Biosyst 4:1075-84 Review
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Atlas JC, Nikolaev EV, Browning ST, Shuler ML (2008) Incorporating genome-wide DNA sequence information into a dynamic whole-cell model of Escherichia coli: application to DNA replication. IET Syst Biol 2:369-82
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Tanner NA, Hamdan SM, Jergic S, Loscha KV, Schaeffer PM, Dixon NE, van Oijen AM (2008) Single-molecule studies of fork dynamics in Escherichia coli DNA replication. Nat Struct Mol Biol 15:998
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Bates D (2008) The bacterial replisome: back on track? Mol Microbiol 69:1341-8 Review
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McGlynn P, Guy CP (2008) Replication forks blocked by protein-DNA complexes have limited stability in vitro. J Mol Biol 381:249-55
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Tang KH, Tsai MD (2008) Structure and function of 2:1 DNA polymerase.DNA complexes. J Cell Physiol 216:315-20 Review
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Espeli O, Mercier R, Boccard F (2008) DNA dynamics vary according to macrodomain topography in the E. coli chromosome. Mol Microbiol 68:1418-27
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Reyes-Lamothe R, Possoz C, Danilova O, Sherratt DJ (2008) Independent positioning and action of Escherichia coli replisomes in live cells. Cell 133:90-102
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Haeusser DP, Levin PA (2008) The great divide: coordinating cell cycle events during bacterial growth and division. Curr Opin Microbiol 11:94-9 Review
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Kouzminova EA, Kuzminov A (2008) Patterns of chromosomal fragmentation due to uracil-DNA incorporation reveal a novel mechanism of replication-dependent double-stranded breaks. Mol Microbiol 68:202-15
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Nielsen O, Løbner-Olesen A (2008) Once in a lifetime: strategies for preventing re-replication in prokaryotic and eukaryotic cells. EMBO Rep 9:151-6 Review
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Tanner NA, Hamdan SM, Jergic S, Loscha KV, Schaeffer PM, Dixon NE, van Oijen AM (2008) Single-molecule studies of fork dynamics in Escherichia coli DNA replication. Nat Struct Mol Biol 15:170-6
- 2007
- 2006
- 1997
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