TopicPage for Mem Proteome Lai2004 of Escherichia coli K-12
| ||Membrane Proteome         |
A study by Lai (2004) identified 28 E. coli proteins from rod and minicell membrane preparations.
They did not separate inner and outer membranes. These 28 proteins are grouped into the subTopic
"Experimentally determined membrane protein localization based on pre-fractionation, two dimensional polyacrylamide gel electrophoresis and identification by Mass Spectroscopy".
Membrane proteins can usually be localized to either the inner or the outer membrane fractions, and
these assignments, based on 2DMS data in other publications, or other types of localization
experiments, or bioinformatics predictions, are noted in the GenePages, so they may be included in other Proteomics subTopics based or other referenced post-genomic studies.
Lai (2004) used minicell/rod-enrichment as a screen for asymmetrically localized membrane proteins,
and further showed polar OmpW and polar/septal YiaF. Lai (2004) washed with alkaline carbonate to reduce
cytoplasmic protein contamination. This may also have removed some membrane-associated proteins.
EM, Nair U, Phadke ND, Maddock JR, (2004) Proteomic screening and
identification of differentially distributed membrane proteins in
Escherichia coli, Mol Microbiol 52:1029-44
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