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TopicPage for Mem Proteome Lai2004 of Escherichia coli K-12

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Membrane Proteome         

Description:

A study by Lai (2004) identified 28 E. coli proteins from rod and minicell membrane preparations. They did not separate inner and outer membranes. These 28 proteins are grouped into the subTopic Mem_Loc_2DMS for
"Experimentally determined membrane protein localization based on pre-fractionation, two dimensional polyacrylamide gel electrophoresis and identification by Mass Spectroscopy".

Details:

Membrane proteins can usually be localized to either the inner or the outer membrane fractions, and these assignments, based on 2DMS data in other publications, or other types of localization experiments, or bioinformatics predictions, are noted in the GenePages, so they may be included in other Proteomics subTopics based or other referenced post-genomic studies.

Lai (2004) used minicell/rod-enrichment as a screen for asymmetrically localized membrane proteins, and further showed polar OmpW and polar/septal YiaF. Lai (2004) washed with alkaline carbonate to reduce cytoplasmic protein contamination. This may also have removed some membrane-associated proteins.

Lai EM, Nair U, Phadke ND, Maddock JR, (2004) Proteomic screening and identification of differentially distributed membrane proteins in Escherichia coli, Mol Microbiol 52:1029-44




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