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TopicPage for G:T mismatch binding of Escherichia coli K-12

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Proteome Chips: Mismatch Binding         

Description:

Chen (2008) identified the top 20 proteins that preferentially bind to dsDNA containing a G:T mismatch, called probe 3. Probe 1 is the same 19-mer without the mismatch on the upper strand.

Details:

5'-GGAATTAAAGTGAAGACC-3'
3'- CCTTAATTTCGCTTCTGG-5

The ratio of the preferential probe 3 binding ranges from 12.2 fold for CspE to 1.7 fold for YfeP. YbcN is ranked second at 7.0 fold.


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