Abasic site:T binding
Proteome Chips: Mismatch Binding
Chen (2008) identified the top 20 proteins that preferentially bind to dsDNA containing an abasic site (N) unpaired to a T, called probe 7. Probe 1 is the same 19-mer with a G:C instead of the T-N.
The ratio of the preferential probe 7 binding ranges from 11.8 fold for YbaZ to 1.8 fold for YcdC. Dgt is ranked second at 7.5 fold.
Chen CS, Korobkova E, Chen H, Zhu J, Jian X, Tao SC, He C, Zhu H (2008) A proteome chip approach reveals new DNA damage recognition activities in Escherichia coli. Nat Methods 5:69-74