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EcogeneTopic Page
TopicPage for RNA degradosome of Escherichia coli K-12
 | Exoribonucleases          |
Description:
The RNA degradosome is a multiprotein complex consisting of RNAse E with PNPase, enolase and RNA helicase RhlB attached to its C-terminal scaffold.
Details:
Alternate forms of the RNA degradosome with different proteins have been reported. Additional alternate degradosome components include PcnB (poly A polymerase) (Raynal, 1999; Carabetta, 2010). Additional alternate degradosome components during cold shock include RNA helicase CsdA (Prud'homme-Genereux, 2004). Additional alternate degradosome components include the RNA helicases RhlE and SrmB (Khemici, 2004). Additional alternate degradosome components during stationary phase include Rnr (RNase R) and the putative RNA helicase HrpA (Carabetta, 2010). Ppk (polyphosphate kinase) has also been reported to be a constiuent of the degradosome (Blum, 1997).
Bibliography (50 total) : Review Only   Up
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- 2010
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Arraiano CM, Andrade JM, Domingues S, Guinote IB, Malecki M, Matos RG, Moreira RN, Pobre V, Reis FP, Saramago M, Silva IJ, Viegas SC (2010) The critical role of RNA processing and degradation in the control of gene expression. FEMS Microbiol Rev 34:883-923 Review
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Carabetta VJ, Silhavy TJ, Cristea IM (2010) The response regulator SprE (RssB) is required for maintaining poly(A) polymerase I-degradosome association during stationary phase. J Bacteriol 192:3713-21
- 2009
- 2008
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Taghbalout A, Rothfield L (2008) New insights into the cellular organization of the RNA processing and degradation machinery of Escherichia coli. Mol Microbiol 70:780-2 Comment
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Worrall JA, Górna M, Crump NT, Phillips LG, Tuck AC, Price AJ, Bavro VN, Luisi BF (2008) Reconstitution and analysis of the multienzyme Escherichia coli RNA degradosome. J Mol Biol 382:870-83
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Richards J, Sundermeier T, Svetlanov A, Karzai AW (2008) Quality control of bacterial mRNA decoding and decay. Biochim Biophys Acta 1779:574-82
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Taghbalout A, Rothfield L (2008) RNaseE and RNA helicase B play central roles in the cytoskeletal organization of the RNA degradosome. J Biol Chem 283:13850-5
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Mackie GA, Coburn GA, Miao X, Briant DJ, Prud'homme-Généreux A, Stickney LM, Hankins JS (2008) Preparation of the Escherichia coli RNase E protein and reconstitution of the RNA degradosome. Methods Enzymol 447:199-213
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Mauri P, Dehò G (2008) A proteomic approach to the analysis of RNA degradosome composition in Escherichia coli. Methods Enzymol 447:99-117
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Carpousis AJ, Khemici V, Aït-Bara S, Poljak L (2008) Co-immunopurification of multiprotein complexes containing RNA-degrading enzymes. Methods Enzymol 447:65-82
- 2007
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Chandran V, Poljak L, Vanzo NF, Leroy A, Miguel RN, Fernandez-Recio J, Parkinson J, Burns C, Carpousis AJ, Luisi BF (2007) Recognition and cooperation between the ATP-dependent RNA helicase RhlB and ribonuclease RNase E. J Mol Biol 367:113-32
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Taghbalout A, Rothfield L (2007) RNaseE and the other constituents of the RNA degradosome are components of the bacterial cytoskeleton. Proc Natl Acad Sci U S A 104:1667-72
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Carpousis AJ (2007) The RNA degradosome of Escherichia coli: an mRNA-degrading machine assembled on RNase E. Annu Rev Microbiol 61:71-87 Review
- 2006
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Marcaida MJ, DePristo MA, Chandran V, Carpousis AJ, Luisi BF (2006) The RNA degradosome: life in the fast lane of adaptive molecular evolution. Trends Biochem Sci 31:359-65
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Durán-Figueroa NV, Piña-Escobedo A, Schroeder I, Simons RW, García-Mena J (2006) Polynucleotide phosphorylase interacts with ribonuclease E through a betabetaalphabetabetaalpha domain. Biochimie 88:725-35
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Chandran V, Luisi BF (2006) Recognition of enolase in the Escherichia coli RNA degradosome. J Mol Biol 358:8-15
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Regonesi ME, Del Favero M, Basilico F, Briani F, Benazzi L, Tortora P, Mauri P, Dehò G (2006) Analysis of the Escherichia coli RNA degradosome composition by a proteomic approach. Biochimie 88:151-61
- 2005
- 2004
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Khemici V, Toesca I, Poljak L, Vanzo NF, Carpousis AJ (2004) The RNase E of Escherichia coli has at least two binding sites for DEAD-box RNA helicases: functional replacement of RhlB by RhlE. Mol Microbiol 54:1422-30
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Prud'homme-Généreux A, Beran RK, Iost I, Ramey CS, Mackie GA, Simons RW (2004) Physical and functional interactions among RNase E, polynucleotide phosphorylase and the cold-shock protein, CsdA: evidence for a 'cold shock degradosome'. Mol Microbiol 54:1409-21
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Morita T, Kawamoto H, Mizota T, Inada T, Aiba H (2004) Enolase in the RNA degradosome plays a crucial role in the rapid decay of glucose transporter mRNA in the response to phosphosugar stress in Escherichia coli. Mol Microbiol 54:1063-75
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Callaghan AJ, Aurikko JP, Ilag LL, Günter Grossmann J, Chandran V, Kühnel K, Poljak L, Carpousis AJ, Robinson CV, Symmons MF, Luisi BF (2004) Studies of the RNA degradosome-organizing domain of the Escherichia coli ribonuclease RNase E. J Mol Biol 340:965-79
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Bernstein JA, Lin PH, Cohen SN, Lin-Chao S (2004) Global analysis of Escherichia coli RNA degradosome function using DNA microarrays. Proc Natl Acad Sci U S A 101:2758-63
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Regonesi ME, Briani F, Ghetta A, Zangrossi S, Ghisotti D, Tortora P, Dehò G (2004) A mutation in polynucleotide phosphorylase from Escherichia coli impairing RNA binding and degradosome stability. Nucleic Acids Res 32:1006-17
- 2002
- 2001
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Liou GG, Jane WN, Cohen SN, Lin NS, Lin-Chao S (2001) RNA degradosomes exist in vivo in Escherichia coli as multicomponent complexes associated with the cytoplasmic membrane via the N-terminal region of ribonuclease E. Proc Natl Acad Sci U S A 98:63-8
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Mackie GA, Coburn GA, Miao X, Briant DJ, Prud'homme-Genereux A (2001) Preparation of Escherichia coli Rne protein and reconstitution of RNA degradosome. Methods Enzymol 342:346-56
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Carpousis AJ, Leroy A, Vanzo N, Khemici V (2001) Escherichia coli RNA degradosome. Methods Enzymol 342:333-45
- 2000
- 1999
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Coburn GA, Miao X, Briant DJ, Mackie GA (1999) Reconstitution of a minimal RNA degradosome demonstrates functional coordination between a 3' exonuclease and a DEAD-box RNA helicase. Genes Dev 13:2594-603
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Lopez PJ, Marchand I, Joyce SA, Dreyfus M (1999) The C-terminal half of RNase E, which organizes the Escherichia coli degradosome, participates in mRNA degradation but not rRNA processing in vivo. Mol Microbiol 33:188-99
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Rauhut R, Klug G (1999) mRNA degradation in bacteria. FEMS Microbiol Rev 23:353-70 Review
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Raynal LC, Carpousis AJ (1999) Poly(A) polymerase I of Escherichia coli: characterization of the catalytic domain, an RNA binding site and regions for the interaction with proteins involved in mRNA degradation. Mol Microbiol 32:765-75
- 1998
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Vanzo NF, Li YS, Py B, Blum E, Higgins CF, Raynal LC, Krisch HM, Carpousis AJ (1998) Ribonuclease E organizes the protein interactions in the Escherichia coli RNA degradosome. Genes Dev 12:2770-81
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Bessarab DA, Kaberdin VR, Wei CL, Liou GG, Lin-Chao S (1998) RNA components of Escherichia coli degradosome: evidence for rRNA decay. Proc Natl Acad Sci U S A 95:3157-61
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