CUG is very rarely used as a start codon in E. coli mRNA.
hda has the only verified CUG start codon that has been published. The predicted CTG start codon for yfjD has been confirmed by start codon mutagenesis (D. Dague and K.E. Rudd, unpublished). The start codon for the pseudogene yghF' is predicted to be CUG, but this cannot be easily verified as yghF' is not expressed (D. Dague and K.E. Rudd, unpublished).
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