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TopicPage for Blue Native 2D PAGE (Lasserre) of Escherichia coli K-12


2D PAGE Protein Complexes         

Description:

Lasserre (2006) identified 50 heteromultimeric (labeled Cmplx1-50 in EcoGene) and 256 homomultimeric (labeled Cmplx51-306 in EcoGene) complexes using two-dimensional blue native/SDS polyacrylamide gel electrophoresis (Blue Native 2D PAGE), followed by spot identification using mass spectrometry methods.

They separated membrane (Mem) and cytoplasmic (Cyt) fractions. The GenePages for the 297 proteins involved in these 306 complexes have links to this TopicPage. Lasserre et al., 2006, refer to "all the protein complexes of the cell" as the complexome, i.e. the end result of interactome of stable protein-protein interactions.

Some proteins were found in more than one complex. This appears to be due, in part, to complexes partitioning to both membrane and cytoplasmic fractions using this procedure The authors point out several limitations of the method, including the identification of some sub-complexes included in this set of E. coli complexes.

Details:

Data for this Topic are derived from Tables in Lasserre (2006).

Homomultimeric Complexes are denoted as HM and Heteromultimeric Complexes are denoted as HT. Cmplx1-50 are heteromultimeric complexes from Table 1 (#1-18, known HT complexes) and Table 2 (#19-50, newly identified HT complexes). Cmplx51-306 are homomultimeric complexes from Table 3 (#230-306, newly identified HM complexes) and the Supplementary Table (#51-229, previously identified as being in an HM complex).

Lasserre JP, Beyne E, Pyndiah S, Lapaillerie D, Claverol S, Bonneu M. , A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis., Electrophoresis. 2006 Jul 21; [Epub ahead of print]

Dr. Marc Bonneu is located at The Functional Genomic Platform at Bordeaux, France.
Dr. Bonneu can be reached at marc.bonneu@estbb.u-bordeaux2.fr


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