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EcogeneTopic Page

TopicPage for CP4-6 of Escherichia coli K-12

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Prophages         

Description:

CP4-6 is a putative defective prophage with 29 genes and 5 pseudogenes (afuB', ykfC', ykfK', ykfL', and ptwF').

Details:

Although identified as a putative prophage-derived element, CP4-6 lacks signature capsid or assembly genes to confirm its origins. Alternatively, it could be derived from a novel integrative plasmid. CP4-6 has apparently inserted into the thrW tRNA gene, creating a 60 bp duplication flanking CP4-6, including 49 bp of duplicated thrW sequence and 11 bp of downstream genomic sequence. The partial thrW fragment marking the clockwise (right) end of CP4-6 is annotated as a DLP12 attachment site thrW-derived pseudogene, named ptfW'. Genomic coordinates: 262182-296489. Length: 34,308 nt

This contiguous prophage interval and its length include compound insertion element sequences that are not prophage genes, IS911A'-IS30A-IS911A'-ISY-IS5A (269430-274373), IS1B-IS30B-ISX (278387-279650) and IS1C (289858-290625). IS1B interrupts the afuB' pseudogene.

The CP4-6 prophage length without the 4944 bp IS911A'-IS30A-IS911A'-ISY-IS5A, the 1264 bp IS1B-IS30B-ISX and the 768 bp IS1C included is 27,332 bp.


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