Reference list for nhaA (EG10652) 97 total  Review Only

• 2010


• Herz K, Rimon A, Olkhova E, Kozachkov L, Padan E (2010) Transmembrane segment II of NhaA Na+/H+ antiporter lines the cation passage, and Asp65 is critical for pH activation of the antiporter. J Biol Chem 285:2211-20



• 2009


• Olkhova E, Kozachkov L, Padan E, Michel H (2009) Combined computational and biochemical study reveals the importance of electrostatic interactions between the "pH sensor" and the cation binding site of the sodium/proton antiporter NhaA of Escherichia coli. Proteins 76:548-59



• Padan E, Kozachkov L, Herz K, Rimon A (2009) NhaA crystal structure: functional-structural insights. J Exp Biol 212:1593-603 Review



• Appel M, Hizlan D, Vinothkumar KR, Ziegler C, Kühlbrandt W (2009) Conformations of NhaA, the Na+/H+ exchanger from Escherichia coli, in the pH-activated and ion-translocating states. J Mol Biol 388:659-72



• Herz K, Rimon A, Jeschke G, Padan E (2009) Beta-sheet-dependent dimerization is essential for the stability of NhaA Na+/H+ antiporter. J Biol Chem 284:6337-47



• Appel M, Hizlan D, Vinothkumar KR, Ziegler C, Kühlbrandt W (2009) Conformations of NhaA, the Na/H exchanger from Escherichia coli, in the pH-activated and ion-translocating states. J Mol Biol 386:351-65



• Dzafić E, Klein O, Screpanti E, Hunte C, Mäntele W (2009) Flexibility and dynamics of NhaA Na+/H+-antiporter of Escherichia coli studied by Fourier transform infrared spectroscopy. Spectrochim Acta A Mol Biomol Spectrosc 72:102-9



• 2008


• Padan E (2008) The enlightening encounter between structure and function in the NhaA Na+-H+ antiporter. Trends Biochem Sci 33:435-43 Review



• Rimon A, Hunte C, Michel H, Padan E (2008) Epitope mapping of conformational monoclonal antibodies specific to NhaA Na+/H+ antiporter: structural and functional implications. J Mol Biol 379:471-81



• Tzubery T, Rimon A, Padan E (2008) Structure-based functional study reveals multiple roles of transmembrane segment IX and loop VIII-IX in NhaA Na+/H+ antiporter of Escherichia coli at physiological pH. J Biol Chem 283:15975-87



• Kedrov A, Appel M, Baumann H, Ziegler C, Muller DJ (2008) Examining the dynamic energy landscape of an antiporter upon inhibitor binding. J Mol Biol 375:1258-66



• 2007


• Hilger D, Polyhach Y, Padan E, Jung H, Jeschke G (2007) High-resolution structure of a Na+/H+ antiporter dimer obtained by pulsed electron paramagnetic resonance distance measurements. Biophys J 93:3675-83



• Beth AH (2007) How to assemble the parts: structures of protein complexes from their components. Biophys J 93:3339



• Rimon A, Tzubery T, Padan E (2007) Monomers of the NhaA Na+/H+ antiporter of Escherichia coli are fully functional yet dimers are beneficial under extreme stress conditions at alkaline pH in the presence of Na+ or Li+. J Biol Chem 282:26810-21



• Arkin IT, Xu H, Jensen MØ, Arbely E, Bennett ER, Bowers KJ, Chow E, Dror RO, Eastwood MP, Flitman-Tene R, Gregersen BA, Klepeis JL, Kolossváry I, Shan Y, Shaw DE (2007) Mechanism of Na+/H+ antiporting. Science 317:799-803



• Olkhova E, Padan E, Michel H (2007) The influence of protonation states on the dynamics of the NhaA antiporter from Escherichia coli. Biophys J 92:3784-91



• Otzen DE, Sehgal P, Nesgaard LW (2007) Alternative membrane protein conformations in alcohols. Biochemistry 46:4348-59



• Kozachkov L, Herz K, Padan E (2007) Functional and structural interactions of the transmembrane domain X of NhaA, Na+/H+ antiporter of Escherichia coli, at physiological pH. Biochemistry 46:2419-30



• 2006


• Kedrov A, Ziegler C, Muller DJ (2006) Differentiating ligand and inhibitor interactions of a single antiporter. J Mol Biol 362:925-32



• Screpanti E, Padan E, Rimon A, Michel H, Hunte C (2006) Crucial steps in the structure determination of the Na+/H+ antiporter NhaA in its native conformation. J Mol Biol 362:192-202



• Kedrov A, Müller DJ (2006) Characterizing folding, structure, molecular interactions and ligand gated activation of single sodium/proton antiporters. Naunyn Schmiedebergs Arch Pharmacol 372:400-12 Review



• Olkhova E, Hunte C, Screpanti E, Padan E, Michel H (2006) Multiconformation continuum electrostatics analysis of the NhaA Na+/H+ antiporter of Escherichia coli with functional implications. Proc Natl Acad Sci U S A 103:2629-34



• Kedrov A, Janovjak H, Ziegler C, Kuhlbrandt W, Muller DJ (2006) Observing folding pathways and kinetics of a single sodium-proton antiporter from Escherichia coli. J Mol Biol 355:2-8



• Saier MH, Tran CV, Barabote RD (2006) TCDB: the Transporter Classification Database for membrane transport protein analyses and information. Nucleic Acids Res 34:D181-6



• 2005


• Padan E, Bibi E, Ito M, Krulwich TA (2005) Alkaline pH homeostasis in bacteria: new insights. Biochim Biophys Acta 1717:67-88 Review



• Zuber D, Krause R, Venturi M, Padan E, Bamberg E, Fendler K (2005) Kinetics of charge translocation in the passive downhill uptake mode of the Na+/H+ antiporter NhaA of Escherichia coli. Biochim Biophys Acta 1709:240-50



• Granseth E, Daley DO, Rapp M, Melén K, von Heijne G (2005) Experimentally constrained topology models for 51,208 bacterial inner membrane proteins. J Mol Biol 352:489-94



• Hilger D, Jung H, Padan E, Wegener C, Vogel KP, Steinhoff HJ, Jeschke G (2005) Assessing oligomerization of membrane proteins by four-pulse DEER: pH-dependent dimerization of NhaA Na+/H+ antiporter of E. coli. Biophys J 89:1328-38



• Hunte C, Screpanti E, Venturi M, Rimon A, Padan E, Michel H (2005) Structure of a Na+/H+ antiporter and insights into mechanism of action and regulation by pH. Nature 435:1197-202



• Daley DO, Rapp M, Granseth E, Melén K, Drew D, von Heijne G (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308:1321-3



• Dibrov P, Rimon A, Dzioba J, Winogrodzki A, Shalitin Y, Padan E (2005) 2-Aminoperimidine, a specific inhibitor of bacterial NhaA Na(+)/H(+) antiporters. FEBS Lett 579:373-8



• 2004


• Padan E, Tzubery T, Herz K, Kozachkov L, Rimon A, Galili L (2004) NhaA of Escherichia coli, as a model of a pH-regulated Na+/H+antiporter. Biochim Biophys Acta 1658:2-13 Review



• Kedrov A, Ziegler C, Janovjak H, Kühlbrandt W, Müller DJ (2004) Controlled unfolding and refolding of a single sodium-proton antiporter using atomic force microscopy. J Mol Biol 340:1143-52



• Galili L, Herz K, Dym O, Padan E (2004) Unraveling functional and structural interactions between transmembrane domains IV and XI of NhaA Na+/H+ antiporter of Escherichia coli. J Biol Chem 279:23104-13



• Tzubery T, Rimon A, Padan E (2004) Mutation E252C increases drastically the Km value for Na+ and causes an alkaline shift of the pH dependence of NhaA Na+/H+ antiporter of Escherichia coli. J Biol Chem 279:3265-72



• 2003


• Venturi M, Hunte C (2003) Monoclonal antibodies for the structural analysis of the Na+/H+ antiporter NhaA from Escherichia coli. Biochim Biophys Acta 1610:46-50 Review



• 2002


• Rimon A, Tzubery T, Galili L, Padan E (2002) Proximity of cytoplasmic and periplasmic loops in NhaA Na+/H+ antiporter of Escherichia coli as determined by site-directed thiol cross-linking. Biochemistry 41:14897-905



• Galili L, Rothman A, Kozachkov L, Rimon A, Padan E (2002) Trans membrane domain IV is involved in ion transport activity and pH regulation of the NhaA-Na(+)/H(+) antiporter of Escherichia coli. Biochemistry 41:609-17



• Venturi M, Seifert C, Hunte C (2002) High level production of functional antibody Fab fragments in an oxidizing bacterial cytoplasm. J Mol Biol 315:1-8



• 2001


• Shijuku T, Saito H, Kakegawa T, Kobayashi H (2001) Expression of sodium/proton antiporter NhaA at various pH values in Escherichia coli. Biochim Biophys Acta 1506:212-7



• Majerník A, Gottschalk G, Daniel R (2001) Screening of environmental DNA libraries for the presence of genes conferring Na(+)(Li(+))/H(+) antiporter activity on Escherichia coli: characterization of the recovered genes and the corresponding gene products. J Bacteriol 183:6645-53



• Wiebe CA, Dibattista ER, Fliegel L (2001) Functional role of polar amino acid residues in Na+/H+ exchangers. Biochem J 357:1-10 Review



• Padan E, Venturi M, Gerchman Y, Dover N (2001) Na(+)/H(+) antiporters. Biochim Biophys Acta 1505:144-57 Review



• Inoue H, Tsuboi Y, Kanazawa H (2001) Chimeric Na(+)/H(+) antiporters constructed from NhaA of Helicobacter pylori and Escherichia coli: implications for domains of NhaA for pH sensing. J Biochem 129:569-76



• Gerchman Y, Rimon A, Venturi M, Padan E (2001) Oligomerization of NhaA, the Na+/H+ antiporter of Escherichia coli in the membrane and its functional and structural consequences. Biochemistry 40:3403-12



• Ravna AW, Sylte I, Dahl SG (2001) Molecular model of the Escherichia coli Na1/H1 antiporter NhaA. Receptors Channels 7:319-28



• Dover N, Padan E (2001) Transcription of nhaA, the main Na(+)/H(+) antiporter of Escherichia coli, is regulated by Na(+) and growth phase. J Bacteriol 183:644-53



• 2000


• Venturi M, Rimon A, Gerchman Y, Hunte C, Padan E, Michel H (2000) The monoclonal antibody 1F6 identifies a pH-dependent conformational change in the hydrophilic NH(2) terminus of NhaA Na(+)/H(+) antiporter of Escherichia coli. J Biol Chem 275:4734-42



• Williams KA (2000) Three-dimensional structure of the ion-coupled transport protein NhaA. Nature 403:112-5



• Steuber J, Schmid C, Rufibach M, Dimroth P (2000) Na+ translocation by complex I (NADH:quinone oxidoreductase) of Escherichia coli. Mol Microbiol 35:428-34



• 1999


• Gerchman Y, Rimon A, Padan E (1999) A pH-dependent conformational change of NhaA Na(+)/H(+) antiporter of Escherichia coli involves loop VIII-IX, plays a role in the pH response of the protein, and is maintained by the pure protein in dodecyl maltoside. J Biol Chem 274:24617-24



• Trchounian A, Kobayashi H (1999) Fermenting Escherichia coli is able to grow in media of high osmolarity, but is sensitive to the presence of sodium ion. Curr Microbiol 39:109-14



• Williams KA, Geldmacher-Kaufer U, Padan E, Schuldiner S, Kühlbrandt W (1999) Projection structure of NhaA, a secondary transporter from Escherichia coli, at 4.0 A resolution. EMBO J 18:3558-63



• Padan E, Gerchman Y, Rimon A, Rothman A, Dover N, Carmel-Harel O (1999) The molecular mechanism of regulation of the NhaA Na+/H+ antiporter of Escherichia coli, a key transporter in the adaptation to Na+ and H+. Novartis Found Symp 221:183-96; discussion 196-9 Review



• 1998


• Padan E, Venturi M, Michel H, Hunte C (1998) Production and characterization of monoclonal antibodies directed against native epitopes of NhaA, the Na+/H+ antiporter of Escherichia coli. FEBS Lett 441:53-8



• Verkhovskaya ML, Barquera B, Verkhovsky MI, Wikström M (1998) The Na+ and K+ transport deficiency of an E. coli mutant lacking the NhaA and NhaB proteins is apparent and caused by impaired osmoregulation. FEBS Lett 439:271-4



• Inoue H, Noumi T, Shimomura T, Takimoto N, Tsuchiya T, Kanazawa H (1998) pH-dependent growth retardation of Escherichia coli caused by overproduction of Na+/H+ antiporter. Biol Pharm Bull 21:1128-33



• Rimon A, Gerchman Y, Kariv Z, Padan E (1998) A point mutation (G338S) and its suppressor mutations affect both the pH response of the NhaA-Na+/H+ antiporter as well as the growth phenotype of Escherichia coli. J Biol Chem 273:26470-6



• Sakuma T, Yamada N, Saito H, Kakegawa T, Kobayashi H (1998) pH dependence of the function of sodium ion extrusion systems in Escherichia coli. Biochim Biophys Acta 1363:231-7



• 1997


• Rothman A, Gerchman Y, Padan E, Schuldiner S (1997) Probing the conformation of NhaA, a Na+/H+ antiporter from Escherichia coli, with trypsin. Biochemistry 36:14572-6



• Carmel O, Rahav-Manor O, Dover N, Shaanan B, Padan E (1997) The Na+-specific interaction between the LysR-type regulator, NhaR, and the nhaA gene encoding the Na+/H+ antiporter of Escherichia coli. EMBO J 16:5922-9



• Blattner FR, Plunkett G, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y (1997) The complete genome sequence of Escherichia coli K-12. Science 277:1453-62



• Rowbury RJ (1997) Regulatory components, including integration host factor, CysB and H-NS, that influence pH responses in Escherichia coli. Lett Appl Microbiol 24:319-28 Review



• Noumi T, Inoue H, Sakurai T, Tsuchiya T, Kanazawa H (1997) Identification and characterization of functional residues in a Na+/H+ antiporter (NhaA) from Escherichia coli by random mutagenesis. J Biochem 121:661-70



• Olami Y, Rimon A, Gerchman Y, Rothman A, Padan E (1997) Histidine 225, a residue of the NhaA-Na+/H+ antiporter of Escherichia coli is exposed and faces the cell exterior. J Biol Chem 272:1761-8



• 1996


• Rothman A, Padan E, Schuldiner S (1996) Topological analysis of NhaA, a Na+/H+ antiporter from Escherichia coli. J Biol Chem 271:32288-92



• Dover N, Higgins CF, Carmel O, Rimon A, Pinner E, Padan E (1996) Na+-induced transcription of nhaA, which encodes an Na+/H+ antiporter in Escherichia coli, is positively regulated by nhaR and affected by hns. J Bacteriol 178:6508-17



• Verkhovskaya ML, Verkhovsky MI, Wikström M (1996) The respiration-driven active sodium transport system in E. coli does not function with lithium. FEBS Lett 388:217-8



• Verkhovskaya ML, Verkhovsky MI, Wikström M (1996) K+-dependent Na+ transport driven by respiration in Escherichia coli cells and membrane vesicles. Biochim Biophys Acta 1273:207-16



• Serrano R (1996) Salt tolerance in plants and microorganisms: toxicity targets and defense responses. Int Rev Cytol 165:1-52 Review



• Rowbury RJ, Hussain NH (1996) Exposure of Escherichia coli to acid habituation conditions sensitizes it to alkaline stress. Lett Appl Microbiol 22:57-61



• 1995


• Rimon A, Gerchman Y, Olami Y, Schuldiner S, Padan E (1995) Replacements of histidine 226 of NhaA-Na+/H+ antiporter of Escherichia coli. Cysteine (H226C) or serine (H226S) retain both normal activity and pH sensitivity, aspartate (H226D) shifts the pH profile toward basic pH, and alanine (H226A) inactivates t J Biol Chem 270:26813-7



• Pinner E, Padan E, Schuldiner S (1995) Amiloride and harmaline are potent inhibitors of NhaB, a Na+/H+ antiporter from Escherichia coli. FEBS Lett 365:18-22



• Inoue H, Noumi T, Tsuchiya T, Kanazawa H (1995) Essential aspartic acid residues, Asp-133, Asp-163 and Asp-164, in the transmembrane helices of a Na+/H+ antiporter (NhaA) from Escherichia coli. FEBS Lett 363:264-8



• Harel-Bronstein M, Dibrov P, Olami Y, Pinner E, Schuldiner S, Padan E (1995) MH1, a second-site revertant of an Escherichia coli mutant lacking Na+/H+ antiporters (delta nhaA delta nhaB), regains Na+ resistance and a capacity to excrete Na+ in a delta microH(+)-independent fashion. J Biol Chem 270:3816-22



• 1994


• Padan E, Schuldiner S (1994) Molecular physiology of the Na+/H+ antiporter in Escherichia coli. J Exp Biol 196:443-56 Review



• Pinner E, Padan E, Schuldiner S (1994) Kinetic properties of NhaB, a Na+/H+ antiporter from Escherichia coli. J Biol Chem 269:26274-9



• Shimamoto T, Inaba K, Thelen P, Ishikawa T, Goldberg EB, Tsuda M, Tsuchiya T (1994) The NhaB Na+/H+ antiporter is essential for intracellular pH regulation under alkaline conditions in Escherichia coli. J Biochem 116:285-90



• Ohyama T, Igarashi K, Kobayashi H (1994) Physiological role of the chaA gene in sodium and calcium circulations at a high pH in Escherichia coli. J Bacteriol 176:4311-5



• Rowbury RJ, Goodson M, Humphrey TJ (1994) Sodium chloride induces an NhaA/NhaR-independent acid sensitivity at neutral external pH in Escherichia coli. Appl Environ Microbiol 60:1630-4



• Carmel O, Dover N, Rahav-Manor O, Dibrov P, Kirsch D, Karpel R, Schuldiner S, Padan E (1994) A single amino acid substitution (Glu134-->Ala) in NhaR1 increases the inducibility by Na+ of the product of nhaA, a Na+/H+ antiporter gene in Escherichia coli. EMBO J 13:1981-9



• Inaba K, Kuroda T, Shimamoto T, Kayahara T, Tsuda M, Tsuchiya T (1994) Lithium toxicity and Na+(Li+)/H+ antiporter in Escherichia coli. Biol Pharm Bull 17:395-8



• 1993


• Dibrov PA (1993) Calcium transport mediated by NhaA, a Na+/H+ antiporter from Escherichia coli. FEBS Lett 336:530-4



• Dibrov PA, Taglicht D (1993) Mechanism of Na+/H+ exchange by Escherichia coli NhaA in reconstituted proteoliposomes. FEBS Lett 336:525-9



• Padan E, Schuldiner S (1993) Na+/H+ antiporters, molecular devices that couple the Na+ and H+ circulation in cells. J Bioenerg Biomembr 25:647-69 Review



• Taglicht D, Padan E, Schuldiner S (1993) Proton-sodium stoichiometry of NhaA, an electrogenic antiporter from Escherichia coli. J Biol Chem 268:5382-7



• Gerchman Y, Olami Y, Rimon A, Taglicht D, Schuldiner S, Padan E (1993) Histidine-226 is part of the pH sensor of NhaA, a Na+/H+ antiporter in Escherichia coli. Proc Natl Acad Sci U S A 90:1212-6



• Pinner E, Kotler Y, Padan E, Schuldiner S (1993) Physiological role of nhaB, a specific Na+/H+ antiporter in Escherichia coli. J Biol Chem 268:1729-34



• 1992


• Ohyama T, Imaizumi R, Igarashi K, Kobayashi H (1992) Escherichia coli is able to grow with negligible sodium ion extrusion activity at alkaline pH. J Bacteriol 174:7743-9



• Yura T, Mori H, Nagai H, Nagata T, Ishihama A, Fujita N, Isono K, Mizobuchi K, Nakata A (1992) Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region. Nucleic Acids Res 20:3305-8



• Rahav-Manor O, Carmel O, Karpel R, Taglicht D, Glaser G, Schuldiner S, Padan E (1992) NhaR, a protein homologous to a family of bacterial regulatory proteins (LysR), regulates nhaA, the sodium proton antiporter gene in Escherichia coli. J Biol Chem 267:10433-8



• 1991


• Karpel R, Alon T, Glaser G, Schuldiner S, Padan E (1991) Expression of a sodium proton antiporter (NhaA) in Escherichia coli is induced by Na+ and Li+ ions. J Biol Chem 266:21753-9



• Thelen P, Tsuchiya T, Goldberg EB (1991) Characterization and mapping of a major Na+/H+ antiporter gene of Escherichia coli. J Bacteriol 173:6553-7



• Taglicht D, Padan E, Schuldiner S (1991) Overproduction and purification of a functional Na+/H+ antiporter coded by nhaA (ant) from Escherichia coli. J Biol Chem 266:11289-94



• 1989


• Padan E, Maisler N, Taglicht D, Karpel R, Schuldiner S (1989) Deletion of ant in Escherichia coli reveals its function in adaptation to high salinity and an alternative Na+/H+ antiporter system(s). J Biol Chem 264:20297-302



• 1988


• Karpel R, Olami Y, Taglicht D, Schuldiner S, Padan E (1988) Sequencing of the gene ant which affects the Na+/H+ antiporter activity in Escherichia coli. J Biol Chem 263:10408-14



• 1987


• Goldberg EB, Arbel T, Chen J, Karpel R, Mackie GA, Schuldiner S, Padan E (1987) Characterization of a Na+/H+ antiporter gene of Escherichia coli. Proc Natl Acad Sci U S A 84:2615-9