Microarray Experiments in Portnoy 2010

ReferenceTopicStrainPlatformCommentsContact
Portnoy 2010Aerobic-Anaerobic Shift E. coli K-12 MG1655 vs. ECOM4LA Affymetrix GeneChip E. coli Genome 2.0 arrays The data represents a genome-wide transcriptome studies of wild-type and ECOM4LA (Escherichia coli cytochrome oxidase mutant 4 (del:cydAB, cyoABCD, cbdAB, and ygiN)) cells under aerobic and anaerobic conditions. Bacterial strains were cultured at 37 degrees C in M9 medium (4 g/L glucose) to OD600=0.6 for the WT and 0.25 for ECOM4LA. Bernhard �. Palsson
4 growth experiments
Exp TypeExp IDExp DataNameVariableShift timeDurationComments
Growth1Show experimental dataWT+O2 vs ECOM4LA+O2ECOM4LA+O2OD600=0.6 WT; OD600=0.25 ECOM4LABacterial strains were cultured at 37 degrees C in M9 medium (4 g/L glucose).
Growth2Show experimental dataWT-O2 vs ECOM4LA-O2ECOM4LA-O2OD600=0.6 WT; OD600=0.25 ECOM4LABacterial strains were cultured at 37 degrees C in M9 medium (4 g/L glucose).
Growth3Show experimental dataWT+O2 vs WT-O2WT-O2OD600=0.6 WT; OD600=0.25 ECOM4LABacterial strains were cultured at 37 degrees C in M9 medium (4 g/L glucose).
Growth4Show experimental dataE4-O2 vs E4+O2E4+O2OD600=0.6 WT; OD600=0.25 ECOM4LABacterial strains were cultured at 37 degrees C in M9 medium (4 g/L glucose).