GenePage for the fruB gene of Escherichia coli K-12

Primary Gene Name: fruB
EcoGene Accession Number: EG10005
K-12 Gene Accession Number: ECK2162
MG1655 Gene Identifier: b2169
Gene Name Mnemonic: Fructose
Alternate Gene Symbols: fpr; fruF
Description: Multiphosphoryl transfer protein: fructose PTS EIIA and FPr; MTP-Fru; diphosphoryl transfer protein (DTP)
  # bp Upstream # bp Downstream
MW: 39647.83 ---------376 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 2262365
Left Intergenic Region

Name: fruK_fruB

Length: 1 bp overlap

Orientation: Codirectional-

Left_end: 2262365

Right_end: 2262365

Centisome: 48.74

Genomic Address
Counterclockwise
Minute or Centisome (%) = 48.74
Right End: 2263495
Right Intergenic Region

Name: fruB_setB

Length: 367 bp gap

Orientation: Divergent

Left_end: 2263496

Right_end: 2263862

Centisome: 48.76

FruB has an N-terminal fructose-specific EIIA domain and a C-terminal HPr-like FPr (fructose-inducible HPr) domain. In the probable fructose-specific PTS kinase cascade, the autophosphorylated enzyme I (PtsI) His189-P transfers a phosphate to His299 in the HPr-like FPr domain of FruB; His299-P transfers a phosphate to His62 of the FruB EIIA domain; His62-P transfers its phosphate to Cys112 of the EIIB domain of FruA and from EIIB to fructose during sugar uptake. 3/70 FruB peptide masses from MS analysis confirm this start codon and indicate that the initial Met is not removed (Maillet, 2007).

Go to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePage

BamHI EcoRI HindIII