GenePage for the cyoB gene of Escherichia coli K-12

Primary Gene Name: cyoB
EcoGene Accession Number: EG10179
K-12 Gene Accession Number: ECK0425
MG1655 Gene Identifier: b0431
Gene Name Mnemonic: Cytochrome o oxidase
Alternate Gene Symbols: None
Description: Cytochrome o oxidase subunit I, quinone-binding domain; cytochrome bo(3) ubiquinol oxidase subunit I
  # bp Upstream # bp Downstream
MW: 74367.91 ---------663 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 448650
Left Intergenic Region

Name: cyoC_cyoB

Length: 11 bp overlap

Orientation: Codirectional-

Left_end: 448650

Right_end: 448660

Centisome: 9.67

Genomic Address
Counterclockwise
Minute or Centisome (%) = 9.67
Right End: 450641
Right Intergenic Region

Name: cyoB_cyoA

Length: 21 bp gap

Orientation: Codirectional-

Left_end: 450642

Right_end: 450662

Centisome: 9.71

Subunit I (CyoB) of cytochrome o(bo3) oxidase contains the high-affinity ubiquinone binding site QH; residues R71, D75, H98 and Q101 are implicated in quinone binding using site-directed mutagenesis; a low-affinity binding site QL is also present, not precisely located but probably near the QH site (MacMillan, 2011; Yap, 2010; Hellwig, 2002). Cytochrome bo undergoes redox-linked structural alterations.

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BamHI EcoRI HindIII