GenePage for the frdA gene of Escherichia coli K-12

Primary Gene Name: frdA
EcoGene Accession Number: EG10330
K-12 Gene Accession Number: ECK4150
MG1655 Gene Identifier: b4154
Gene Name Mnemonic: Fumarate reductase
Alternate Gene Symbols: None
Description: Fumarate reductase flavoprotein subunit
  # bp Upstream # bp Downstream
MW: 65971.60 ---------602 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 4380510
Left Intergenic Region

Name: frdB_frdA

Length: 8 bp overlap

Orientation: Codirectional-

Left_end: 4380510

Right_end: 4380517

Centisome: 94.37

Genomic Address
Counterclockwise
Minute or Centisome (%) = 94.37
Right End: 4382318
Right Intergenic Region

Name: frdA_epmA

Length: 324 bp gap

Orientation: Divergent

Left_end: 4382319

Right_end: 4382642

Centisome: 94.41

Purified FrdAB has fumurate reductase activity and has low level succinate dehydrogenase activity (Dickie, 1979). FrdA was shown to be covalently modified with an FAD linked through the methyl group at position 8alpha to the N(3) of one of the FrdA histidine residues (Weiner, 1979). His45 was predicted to be the site of FrdA flavinylation with FAD by similarity to the sequenced histidine-modified bovine heart flavopeptide SHTVAAXGGIBLAAGBMDEBXWR (Kenney, 1972; Cole, 1982). Substitutions of FrdA at His45 still allow tight binding of FAD non-covalently but nonetheless still result in defective enzyme activities (Blaut, 1989). In vitro flavinylation of FrdA using crude extracts was stimulated by citrate, isocitrate, succinate and fumarate (Brandsch, 1989). The crystal structure shows that His45 of FrdA is covalently modified with FAD to form tele-8alpha-FAD histidine (Iverson, 1999). SdhE, the SdhA flavinylator, may also facilitate FrdA flavinylation (McNeil, 2013). The FrdA N-terminus is blocked so it could not be protein sequenced to verify the start; if the fMet is clipped then the Glu2 could form a blocking pyroglutamate, J. H. Weiner, personal communication cited in (Cole, 1982). frdA transcription is repressed by nitrate via the NarXL system. Repression is both direct by repressing promoter activity and indirect by repressing dcuS promoter activity. FrdAB and OmpW form a complex (Huang, 2006). HT_Cmplx15_Mem: FrdA+FrdB.

Go to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePage

BamHI EcoRI HindIII