GenePage for the glpR' pseudogene of Escherichia coli K-12

Primary Gene Name: glpR'
EcoGene Accession Number: EG10400
K-12 Gene Accession Number: ECK3409
MG1655 Gene Identifier: b3423
Gene Name Mnemonic: Glycerol phosphate
Alternate Gene Symbols: None
Description: Pseudogene, repressor of glp regulon; induced by glycerol phophate; tetrameric
  # bp Upstream # bp Downstream
Verified Start MW: 28047.84 ---------252 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 3559848
Left Intergenic Region

Name: rtcR_glpR

Length: 19 bp overlap

Orientation: Convergent

Left_end: 3559848

Right_end: 3559866

Centisome: 76.69

Genomic Address join

The following join statement uses GenBank syntax to describe how the reconstructed frameshifted glpR pseudogene protein sequence was translated from the DNA:

complement(join(3559848..3560453,3560456..3560605))


Counterclockwise
Minute or Centisome (%) = 76.69
Right End: 3560605
Right Intergenic Region

Name: glpR_glpG

Length: 16 bp gap

Orientation: Codirectional-

Left_end: 3560606

Right_end: 3560621

Centisome: 76.71

The glpR pseudogene in MG1655(Seq) is a lab mutation with a frameshift mutation (deletion of G at codon 51) acquired during the genome sequencing process; normal MG1655 strains have an intact glpR gene (Freddolino, 2012). The G at codon 51 has been reinserted in the glpR pseudogene reconstruction displayed by the DNA button; the protein tab displays the reconstructed GlpR protein sequence; the DNA tab shows the unreconstructed genomic DNA sequence for glpR'.

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BamHI EcoRI HindIII