GenePage for the gltB gene of Escherichia coli K-12

Primary Gene Name: gltB
EcoGene Accession Number: EG10403
K-12 Gene Accession Number: ECK3202
MG1655 Gene Identifier: b3212
Gene Name Mnemonic: Glutamate
Alternate Gene Symbols: aspB; ossB; psiQ
Description: Glutamate synthase, large subunit; autoprotease
  # bp Upstream # bp Downstream
Verified Start MW: 163296.99 ---------1486 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 3354725
Left Intergenic Region

Name: yhcC_gltB

Length: 674 bp gap

Orientation: Divergent

Left_end: 3354051

Right_end: 3354724

Centisome: 72.26

Genomic Address
Clockwise
Minute or Centisome (%) = 72.27
Right End: 3359185
Right Intergenic Region

Name: gltB_gltD

Length: 12 bp gap

Orientation: Codirectional+

Left_end: 3359186

Right_end: 3359197

Centisome: 72.37

The N-terminal 11 aa propeptide of GltB is processed autolytically and the mature protein starts at residue 12, as confirmed by N-terminal protein sequencing; an alternate start codon 93 bases upstream has been noted (Oliver, 1987). Although this upstream gltB start codon is preceded by an RBS sequence, the upstream protein sequence is not conserved and is interrupted by stop codons in Salmonella and other closely related bacteria. Cys12 acts as both the proteolytic nucleophile in the autocleavage event and the N-terminal cysteine active site for the glutamine amidotransferase activity; the 11 aa propeptide autocleavage is the only known proteolytic activity of the MEROPS family C44 proteases and the mature C44 proteases are devoid of protease activity (Rawlings, 2014). The yhcC-gltBDF divergent operon promoter region binds ArcA (Federowicz, 2014).

Go to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePage

BamHI EcoRI HindIII