GenePage for the hemB gene of Escherichia coli K-12

Primary Gene Name: hemB
EcoGene Accession Number: EG10428
K-12 Gene Accession Number: ECK0366
MG1655 Gene Identifier: b0369
Gene Name Mnemonic: Hemin
Alternate Gene Symbols: ncf
Description: 5-Aminolevulinate dehydratase; also known as porphobilinogen synthase; binds Zn(II)
  # bp Upstream # bp Downstream
Verified Start MW: 35624.79 ---------324 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 388753
Left Intergenic Region

Name: tauD_hemB

Length: 106 bp gap

Orientation: Convergent

Left_end: 388647

Right_end: 388752

Centisome: 8.37

Genomic Address
Counterclockwise
Minute or Centisome (%) = 8.38
Right End: 389727
Right Intergenic Region

Name: hemB_yaiT

Length: 523 bp gap

Orientation: Divergent

Left_end: 389728

Right_end: 390250

Centisome: 8.40

The hemB auxotropic strain RP523 has unmapped mutation(s) that make the cells permeable to heme, forming the basis of an expression system for heme substitution (Li, 1988; Woodward, 2007; Winter, 2013).

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BamHI EcoRI HindIII