GenePage for the crl' pseudogene of Escherichia coli K-12

Primary Gene Name: crl'
EcoGene Accession Number: EG11092
K-12 Gene Accession Number: ECK0241
MG1655 Gene Identifier: b0240
Gene Name Mnemonic: Curli
Alternate Gene Symbols: None
Description: Pseudogene, sigma factor-binding protein; stimulates RNAP holoenzyme formation; stimulates RpoS activity during stationary phase; mutants display rpoS mutant phenocopy; mutant does not have reduced amount of RpoS protein
  # bp Upstream # bp Downstream
Verified Start MW: 15654.99 ---------133 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 257829
Left Intergenic Region

Name: frsA_crl

Length: 57 bp gap

Orientation: Codirectional+

Left_end: 257772

Right_end: 257828

Centisome: 5.55

Genomic Address join

The following join statement uses GenBank syntax to describe how the reconstructed IS-interrupted crl pseudogene protein sequence was translated from the DNA:

join(257829..257899,258676..259006)


Clockwise
Minute or Centisome (%) = 5.55
Right End: 259006
Right Intergenic Region

Name: crl_insB9

Length: 23 bp gap

Orientation: Convergent

Left_end: 257900

Right_end: 257922

Centisome: 5.56

RpoD, RpoS and RpoH sigma factor-containing holoenzyme assembly is stimulated by Crl (Gaal, 2006). Accumulation of Crl during stationary phase at 30C, but not 37C, suggests it is a thermosensor controlling sigma(S) activity. Initially identified due to reduction of RpoS-dependent curlin (csgAB) expression. The crl IS1I insertion after codon 33 creating a pseudogene in MG1655(Seq) (ATCC 700926; CGSC 7740) is a lab mutation acquired during the genome sequencing process; the parental MG1655 strain (ATCC 47076; CGSC 6300) has an intact crl gene (Freddolino, 2012).

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BamHI EcoRI HindIII