GenePage for the asmA gene of Escherichia coli K-12

Primary Gene Name: asmA
EcoGene Accession Number: EG11361
K-12 Gene Accession Number: ECK2058
MG1655 Gene Identifier: b2064
Gene Name Mnemonic: Assembly suppressor mutant
Alternate Gene Symbols: yegA
Description: Suppressor of OmpF assembly mutants; inner membrane-anchored periplasmic protein; putative outer membrane protein assembly factor; required for swarming phenotype
  # bp Upstream # bp Downstream
MW: 68962.84 ---------617 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 2139759
Left Intergenic Region

Name: yegH_asmA

Length: 273 bp gap

Orientation: Convergent

Left_end: 2139486

Right_end: 2139758

Centisome: 46.09

Genomic Address
Counterclockwise
Minute or Centisome (%) = 46.10
Right End: 2141612
Right Intergenic Region

Name: asmA_dcd

Length: 21 bp gap

Orientation: Codirectional-

Left_end: 2141613

Right_end: 2141633

Centisome: 46.14

Protein fractionation indicates that AsmA is associated with the inner membrane (Deng, 1996). SignalP and TMHMM indicate a single N-terminal transmembrane segment of AsmA may serve as a signal anchor. Thus AsmA is predicted to be mostly periplasmic, consistent with its role in outer membrane assembly of OmpF. Although no association of AsmA with the OM was detected, AsmA superfamily members YhjG and YicH (but not AsmA itself) were predicted to be beta-barrel proteins indicating that the inner membrane-anchored AsmA family proteins may also insert into the outer membrane, consistent with their putative role in OM protein assembly and OM maintenance (Zhai, 2009).

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BamHI EcoRI HindIII