GenePage for the gltF gene of Escherichia coli K-12

Primary Gene Name: gltF
EcoGene Accession Number: EG11514
K-12 Gene Accession Number: ECK3204
MG1655 Gene Identifier: b3214
Gene Name Mnemonic: Glutamate
Alternate Gene Symbols: ossB
Description: Periplasmic protein, function unknown
  # bp Upstream # bp Downstream
Verified Start MW: 26351.68 ---------254 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 3361176
Left Intergenic Region

Name: gltD_gltF

Length: 559 bp gap

Orientation: Codirectional+

Left_end: 3360617

Right_end: 3361175

Centisome: 72.40

Genomic Address
Clockwise
Minute or Centisome (%) = 72.41
Right End: 3361940
Right Intergenic Region

Name: gltF_yhcA

Length: 171 bp gap

Orientation: Codirectional+

Left_end: 3361941

Right_end: 3362111

Centisome: 72.43

A gltF mutant has no phenotype with respect to amino acid utilization or ammonium transport; previous suggestions that GltF has a role in nitrogen-regulated gene expression are now unlikely. Although the GltF protein was overproduced using foreign translation signals, the detection of a signal peptide cleavage verifies the mature protein N-terminus. The precursor was also sequenced and the initial Met (MF) is not cleaved. The yhcC-gltBDF divergent operon promoter region binds ArcA (Federowicz, 2014).

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BamHI EcoRI HindIII